Intro Cells and Microscopy

Question 1

What is an organism

Answer 1

Defined as an assembly of molecules function as a stable whole that exhibits life

Question 2

What is biology

Answer 2

The study of life “bios”=life “logy”=the study of

Question 3

What is the cell theory

Answer 3

1.All organisms are composed of one or more cells
2.The cell is the basic structural function al unit of all living things
3. Cells only arise from preexisting cells

Question 4

what basic features of cells structure are shared by all living cells

Answer 4

1. Plasma Membrane
2. Ribosomes
3. DNA
   4.Cytoplasm

Question 5

How are cells visualized?

Answer 5

Using a microscope

Question 6

What is magnification

Answer 6

the ratio of the object’s image size to its real size (e.g., 1200X)

Question 7

What is resoloution

Answer 7

is a measure of the clarity of the images; it is the minimum
distance that two points in the specimen can be separated and
still be seen as two points Depends primarily on the wavelength of light or electrons used to
illuminate the specimen

Question 8

Light microscopes

Answer 8

Use light to illuminate the specimen. ex, reflected light transmitted light, fluoresences

Question 9

Electron Microscopes

Answer 9

use electrons to illuminate the specimen ex,Transmission, scanning

Question 10

Light microscopy

Answer 10

• Bright field microscopy
• Differential interference
  contrast microscopy (DIC)
  -Fluorescence microscopy
  -Confocal microscopy

Question 11

Electron Microscopy:

Answer 11

-Transmission electron
microscopy (TEM)
– Scanning electron
microscopy (SEM)

Question 12

Confocal Laser Scanning Microscopy
advantages

Answer 12

Live cell imaging
* Real time imaging
* Multicolor imaging
* High-resolution and less background in thick tissues
* 3-D imaging