Intro and Microscopy/ Staining Flashcards
Micro Final
Antoni van Leeuwenhoek
made and used simple microscopes, recognized microorganisms
Carolus Linnaeus
developed Taxonomy: classification
Louis Pasteur
Spontaneous generation did not occur
germ theory, fermentation
3 ways to get rid or microorganisms: filter, heat, expose to chemicals
Robert Koch
originated use of petri dish and using agar
link certain organisms with disease
Christian Gram
developed gram stain
Ignaz Semmelweis
noticed death rates were higher in maternity wards staffed by med students than by midwives
Joseph Lister
father of modern asepsis
Florence Nightingale
cleanliness in nursing and public health
John Snow
public hygiene and infection control
Edward Jenner
immunology, made incisions with cowpox to prevent smallpox
Paul Ehrlich
differential staining of blood cells
chemotherapy, defined cell types
Taxonomy
hierarchical scheme for the classification, identification, and nomenclature of organisms
Taxa
categories organisms are grouped into based on features
Prokaryotes vs. Eukaryotes
P: bacteria, archaea
E: Fungi, protozoa, algae
Viruses
Acellular, cannot reproduce, cannot make ATP, must take over host
Binomial scientific names
Genus- CAPITALIZED
Species- lowercase
both underlined or italicized
Microscope parts
Parfocal
object viewed under low power should still be in view under high power
How to determine total Mag.
magnification of lens x magnification of ocular lens
Oil immersion function
more magnification
Why do we heat fix slides?
kills organisms, adheres them to slide, alters organism so they can readily accept dyes
Simple Stain
single positively charged dye
Acid-fast stain
way to stain cells with mycolic acid in their cell walls
Chemicals in a Gram stain
crystal violet
iodine
decolorization
safranin
