Intermediate filaments Flashcards
Which types of animals have intermediate filaments (IFs)?
Vertebrates and soft bodied metazoans, all animals but sponges.
What function do intermediate cells have? Given that, where are they likely to be found in abundance?
They are structural rope like structures, they are extremely strong. They are fond in cells under a great deal of mechanical stress.
What gives the structural strength to cells in tendons and ligaments?
The intermediate filaments.
How many kinds of IFs do insects have? Why?
1 type, they have chitin to provide structural support.
Keratin is made of what?
Keratin is the IFs found in claws, skin, and hair. Repeat Keratin is intermediate filaments
Where are IFs found?
The cytoplasm and the nucleus
What is Vimentin?
An intermediate filament in the cytosol (cytosolic skeleton)
Lamin B (or any other lamin) found where do what?
Nucleus, they are part of the nuclear lamina. An IF structure that provides integrity to the nucleus and is involved in regulation of cell cycle through phosphorylation.
What size is... Intermediate filaments Actin Microtubules myosin
Intermediate filaments: 10 nM
Actin: 7-9 nM
Microtubules: 25 nM
myosin 20 nM
what else are IFs known as?
Nano filaments.
Go through Intermediate filament formation
- Monomers have alpha helices.
- A monomer raps around another in a parallel coiled coil arrangement, forming a dimer
- A dimer aligns slightly offset, by another dimer, in an antiparallel arrangment forming a tetramer
- 8 tetramers twist together to form a rope type filament
What type of contact lateral or lengthwise dominate in intermediate filaments?
Intermediate filaments will contact the filaments running alongside them far more then those next to them, but since they are staggered, they are all strongly interconnected.
Rank in terms of stress befroe fu
actin
microtubules
intermediate
actin: 33
microtubules ruptures: 3
intermediate: 60 not yet ruptured
What types of proteins do IFs have?
They have cross-linking and bundling proteins.
which types of proteins do they lack?
They lack nucleating, sequestering, capping, and severing proteins.
IFs are organized into higher order structures called ______, which is achieved by using ______. This increases strength and stability.
Strong arrays.
Crosslinking and bundling proteins.
Neurofilaments NF-M and NF-H do what differently then normal IFs?
They have an elongated C terminus, which binds to neighbors allowing them to self bundle.
Specific to nerve cells.
Name two major accessory proteins for IFs and what they interact with.
Filaggrin: keratin
Plectin: vimentin
What does Filaggrin do?
Is likely a bundling protein which binds keratin, helps increase toughness of skin, which has a lot of keratin.
What does plectin do?
Plectin bundles vimentin by cross-linking the filaments. Mutations of plectin can be lethal.
Can IFs be modified after they are constructed?
No. They are stable once constructed, which is a major difference between IFs and microtubules and actin.
What does it mean when I say that IFs are more heterogenous then actin and microtubules?
There are more kinds of IFs.
Do IFs? Interact with motor proteins? Have intrinsic polarity? Bind ATP or GTP? Have enzymatic function?
Interact with motor proteins? No Have intrinsic polarity? No Bind ATP or GTP? No Have enzymatic function? No
How many genes encode for IFs? How many disorders are associated with defects in IFs?
70 genes.
40 disorders.
Four broad types of IFs
Nuclear
Vimentin Like
Epithelial
Axonal
Nuclear IFs
Lamins A, B, C inner lining of nucleus