Instrumentation Quiz 2

Question 0

What color light on the DXI indicates supplies required. One or more system supply areas are low or waste containers are almost full. The system will continue to process samples and schedule new tests

Answer 0

Amber, steady

Question 1

What color light indicates the DXI is either stopped or that initialization is in process

Answer 1

Red

Question 2

What color on the DXI indicates supplies are required and one or more system supplies are out or an area requires attention. The system will not schedule the test but will continue test in progress?

Answer 2

Amber blinking

Question 3

What color light on the DXI means the system is running, processing test, or performing routine maintenance

Answer 3

Green

Question 4

What color light on the DXI indicates the instrument is ready, no processing but the system is ready to begin processing

Answer 4

Blue

Question 5

What occurs during reagent addition on the DXI?

Answer 5

Reagent pipettor transfers specified quantities of reagents into the reaction vessel. Robotic module moves the RV to the incubator. Paramagnetic particles from the reagents bind with the analyte

Question 6

What occurs during sample washing on the DXI

Answer 6

RV is moved to wash carousel and wash buffer is added to the RV. Magnets draw paramagnetic particles to the side of the reaction vessel and an aspirin probe removes wash buffer and unbound analyte

Question 7

What is the purpose of the substrate probe on the DXI

Answer 7

To dispense chemiluminescent substrate into the RV

Question 8

Which assay on the Centar uses AE labeled antibodies and solid phase para magnetic particles that are coated with specific anti-body for the antigen in the sample?

Answer 8

Sandwich assay

Question 9

What is the proportionality between chemiluminescent and analyte concentration for the sandwich assay

Answer 9

Direct

Question 10

Which assay on the Centar uses AE labeled antigen and anti-body labeled paramagnetic particles?

Answer 10

Competitive

Question 11

What is the proportionality between chemiluminescence and analyte concentration for the competitive assay

Answer 11

Inverse

Question 12

Which type of essay on the Centar uses a solid phase containing anti-human IgM anti-body bound to para magnetic particles and AE labeled antigen?

Answer 12

Antibody capture

Question 13

What is the proportionality between chemiluminescence and analyte concentration for the anti-body capture assay

Answer 13

Direct

Question 14

What is the purpose of nitric acid for a sweat chloride analysis

Answer 14

To provide a good electrical conductivity

Question 15

What is the purpose of acetic acid in a sweat chloride procedure

Answer 15

To make silver chloride insoluble by making the solution less polar and to yield a sharp end point

Question 16

What is the purpose of gelatin in the sweat chloride procedure

Answer 16

To provide a uniform current flow by equalizing high spots on the electrode

Question 17

What is the expected range for a sweat chloride on patients older than six months

Answer 17

0 to 39 mmol/ L

Question 18

What is the expected range for a sweat chloride for patients up to and including six months of age

Answer 18

0 to 29 mmol per L

Question 19

What is the abnormal critical value of a sweat chloride

Answer 19

> or equal to 60

Question 20

What is the indeterminate range for patients older than six months on a sweat chloride

Answer 20

40-59 mmol per L

Question 21

What is the indeterminate range for patients up to and including six months of age on the sweat chloride

Answer 21

30-59

Question 22

What are five symptoms that a patient will exhibit that might cause a physician to order a sweat chloride

Answer 22

Difficulty breathing, wheezing, cough, failure to thrive, greasy stools, liver disease, nasal polyps

Question 23

What are common causes of agents for infections present and cystic fibrosis patients

Answer 23

Staph aureus and Pseudomonas aeruginosa