Inhibition Of Protein Synthesis By Antibiotics

Question 1

Puromycin

Answer 1

• euk and prok (NOT clinically useful)

- premature termination by binding to A site and linking with petidyl tRNA on P site

Question 2

Cycloheximide

Answer 2

Euk (only used for research)

-interacts w/ 60S and prevents peptidyl transferase activity

Question 3

Chloramphenicol

Answer 3

• prok and mitochondria
• used for severe infection only b/c it inhibits euk mitochondrial protein synthesis
• inhibits peptidyl transferase activity by interacting w/ 23S of 50S

Question 4

Streptomycin

Answer 4

• Prok

- bind to 30S and inhibits initiation/causes misreading of mRNA

Question 5

Tetracyclines

Answer 5

• prok

- interacts w/ 30S and inhibits binding of incoming AA-tRNA to A site

Question 6

Erythromycin

Answer 6

• Prok

- interacts w/ 50S and blocks exit tunnel/release of nascent polypeptide