Infiltration & Embedding Flashcards
The process of completely removing the clearing agent and replacing it with a medium that will completely fill all the tissue cavities
Infiltration /impregnation
Purpose of infiltration and embedding?
Give firm consistency and allow easier handling and cutting
Other term for embedding
Blocking/casting
T/F
The medium used in infiltration is almost always the same with the medium used in embedding
True
Simplest and most common embedding medium
Paraffin wax
Allow serial section cutting
Paraffin wax
Melting point of paraffin wax
56-57*C
Paraffin can’t infiltrate
BeeBee
Bones
Teeth
brain
eyes
This EM is not recommended for fatty tissues
Paraffin wax
Overheated paraffin will cause
Brittleness
Too hot = marupok
Melting points of common waves
45, 52, 56, 58*C
Recommended MP of paraffin waxes according to lab temp
20-24*C = 54-58*C 15-18*C = 50-54 *C
T/F
👆🏻melting point = harder wax = easier cutting
True
T/F
The harder the wax the more thinner the sections it can produce
True
T/F
Harder tissue require harder wax
True