Infiltration & Embedding Flashcards

1
Q

The process of completely removing the clearing agent and replacing it with a medium that will completely fill all the tissue cavities

A

Infiltration /impregnation

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2
Q

Purpose of infiltration and embedding?

A

Give firm consistency and allow easier handling and cutting

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3
Q

Other term for embedding

A

Blocking/casting

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4
Q

T/F

The medium used in infiltration is almost always the same with the medium used in embedding

A

True

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5
Q

Simplest and most common embedding medium

A

Paraffin wax

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6
Q

Allow serial section cutting

A

Paraffin wax

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7
Q

Melting point of paraffin wax

A

56-57*C

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8
Q

Paraffin can’t infiltrate

A

BeeBee

Bones
Teeth
brain
eyes

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9
Q

This EM is not recommended for fatty tissues

A

Paraffin wax

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10
Q

Overheated paraffin will cause

A

Brittleness

Too hot = marupok

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11
Q

Melting points of common waves

A

45, 52, 56, 58*C

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12
Q

Recommended MP of paraffin waxes according to lab temp

A
20-24*C = 54-58*C
15-18*C = 50-54 *C
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13
Q

T/F

👆🏻melting point = harder wax = easier cutting

A

True

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14
Q

T/F

The harder the wax the more thinner the sections it can produce

A

True

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15
Q

T/F

Harder tissue require harder wax

A

True

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16
Q

3 ways of paragon wax infiltration

A

Manual (4)
Automatic (2-3)
Vacuum (3)

17
Q

Give the needed temp for infiltration in each method

A

Manual - 2-5C
Automatic - 3
C
Vacuum -2-4*C

18
Q

Other term for automatic tissue processor

A

Autotechnicon

19
Q

T/F

Autotechnicon has vacuum which makes impregnation faster

A

False

-constant agitation

20
Q

How many breakers are used in autotechnicon?

A

12
10- glass
2- thermostatically controlled wax baths

21
Q

This method involves wax impregnation under negative atmospheric pressure (vacuum)

A

Vacuum embedding

22
Q

Most rapid method

A

Vacuum

-reduce 25-75% of the normal time required for tissue processing

23
Q

Factors affecting paraffin wax impregnation

A
  1. Nature & size of tissue

2. Type of clearing agent

24
Q

Temp above _______ temp will cause considerable damage to tissue

A

60*C

25
Q

What to do before using paraffin

A

Melt at 2*C above MP then filter using Green’s no. 904

26
Q

What to if if paragon wax is contaminated with water?

A

Heat 100-105*C

27
Q

Mediums that can be used in impregnation

A

Paraffin
Celloidin
Gelatin

28
Q

Paraffin should be melted at what temp?

A

5-10*C above MP

29
Q

The process by which tissue is arranged in a precise position

A

Orientation

30
Q

3 ways of solidifying the block

A

Ref @ 5*C
Cold water
Cold plate