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Histopathology Lab > All About Fixatives > Flashcards

All About Fixatives Flashcards

1
Q

Satisfactory ph

A

Ph 6-8

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2
Q 
Fixation temp of:
Tissue processors?
Electron microscopy and histochemistry?
Urgent biopsy specimens?
Tissue with tuberculosis?
A

40C
0-4C
60C
100C

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3
Q

Thickness of section
Electron microscopy?
Light microscopy?

A

1-2 mm^2

2 cm^2

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4
Q

Brain is suspended whole in what formalin?

A

10% buffed formalin for 2-3 weeks

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5
Q

Osmolality that gives the best result?

A

Slightly hypertonic solution (400-450 mOsm)

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6
Q

Concentration usually used for
Formalin?
Formaldehyde?

A

10%

3%

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7
Q

Volume of fixative to tissue

A

20:1

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8
Q

Fixation time for formalin

A

24 hrs

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9
Q

Prolonged fixation May cause?

A
  • shrinkage

- gardening severely inhibit enzyme activity and immunological reactions

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10
Q

Duration of fixation for electron microscope?

A

3 hrs

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11
Q

Penetration rate of formalin

A

1 mm/hr (slows down as it goes deeper)

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12
Q

Fixation time an be cut down by using?

A

Heat, vacuum, agitation or microwave

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13
Q

Recommended size of tissue?

A

2 cm^2 and no more than 4mm thick

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14
Q

All tissues can be cut and trimmed without prior fixation except?

A

Brain

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15
Q

T/F

Osmium inhibits hematoxylin staining

A

True

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16
Q

Fixatives made up of only 1 component

A

Simple fixatives

17
Q

Fixatives made up of two or more fixatives?

A

Compound fixatives

18
Q

Name the simple fixatives

A

MALH

Metallic
Aldehydes
Lead
Heat

19
Q

Types of fixatives according to action?

A
  • microanatomical

- cytological

20
Q

Fixative used for lipid

A

Fixatives containing:

  • mercuric chloride
  • potassium dichromate
21
Q

Used for glycogen fixation?

A

Alcoholic fixatives

22
Q

Used for protein fixation

A
  • neutral buffered formol saline

- formaldehyde Vapors

23
Q

Type of fixatives that are satisfactory for routine paraffin sections

A

Aldehyde fixatives

24
Q

Ideal size of specimen

A

3mm

-should not be more than 4mm think

25
Q

T/F

👆🏻temp = 👆🏻rate of diffusion = 👆🏻 rate of chemical rxn

A

True

26
Q

Fixatives penetrate by?

A

Diffusion

27
Q

Highly polymerised form of formaldehyde?

A

Paraformaldehyde

28
Q

Alcohol fixatives conc range?

A

60-100

-less conc will lyse the cell

29
Q

Methanol 100%

A

Can fix: dry and wet smears, blood smears, bone marrow tissues

30
Q

Chemicals that fixes and dehydrates at the same time

A

Methanol

Acetone

Histopathology Lab (6 decks)

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