Dehydration

Question 1

Process of removal of fixative and water from tissue

Answer 1

Dehydration

Question 2

T/F

The process is carried out by submerging the specimen in decreasing conc of alcohol

Answer 2

FALSE

Question 3

Ratio of dehydrating agent to tissue

Answer 3

10:1

Question 4

Most commonly used chemical

Answer 4

Ethanol

Question 5

Strength of initial alcohol conc depends on?

Answer 5

1. Size of specimen
2. Nature
   3 fixative used

Question 6

T/F

After fuxatuon, submerge embryonic tissue to 70% alcohol then 95% then 100%

Answer 6

FALSE

- start with 30% for delicate tissue

Question 7

Submerging delicate tissue directly to high grades of alcohol will cause

Answer 7

Tissue Hardening, shrinkage, brittleness

Question 8

A temp of _________ will hasten dehydration

Answer 8

37*C

Question 9

Addition of what chemical will remove water from dehydrating agent and will turn blue

Answer 9

Anhydrous copper sulfate

Question 10

This chemical is combustible at 110-120*C

Answer 10

Cellosolve

Question 11

Chemicals can be used in dehydration and clearing

Answer 11

Dioxane

Tetrahydrofuran

Question 12

Can cause explosion in the lab due to explosive peroxides

Answer 12

Dioxane (die)

Question 13

Chemical that can improve staining results

Answer 13

Tetrahydrofuran

Question 14

Acts as a softener for hard tissue like: tendon, nail, dense fribrous tissue

Answer 14

4% phenol

Question 15

Hydrophilic/hydrophobic

Melted paraffin wax

Answer 15

Hydrophobic

-thus, there is a need to remove water from the tissue in order for the paraffin to completely penetrate the tissue