Induction

Question 1

Neural induction

Answer 1

The process by which embryonic cells in the ectoderm make a decision to acquire a neural fate rather than give rise to mesoderm or epidermis

Question 2

Neural induction transforms…

Answer 2

The neurogenic region into the neural plate which then undergoes a series of morphogenic movements to form the neural tube and notochord

Question 3

Hilda mangold

Answer 3

Took dorsal lip from pigmented newt and transplanted into non-pigmented

Resulted in duplicated axis
2nd axis not pigmented: sequestered cells from host

Question 4

Zebrafish embryo contains…

How do cells move?

Answer 4

Massive yolk
Acts as a scaffold

Maternal determinants move to top of embryo and form one large cell that divides and moves down yolk

Stops at about 59% for gastrulation

Question 5

Zebrafish shield transplant

Answer 5

Inject GPF into yolk
Transplant shield into ventral side of host

Duplicated axis, one tail

There is a secondary/tail organiser, if this is also transplanted= two tails

Question 6

Conservation

Answer 6

Organisers well conserved across species

Chicken organiser can duplicate axis in zebrafish

Question 7

Animal caps (dorsal ectoderm) isolated _______ to ________ and cultured give rise to ________.

The same tissue from ___________ embryos gives rise to _______________.

Difference?

Answer 7

Prior
Gastrulation
Epidermis

Gastrulating
Neuronal tissue

Mesoderm has been induced

Question 8

Removing the animal cap causes?

Answer 8

Failure to develop mesoderm

Question 9

Mesoderm generation requires:

Answer 9

Interaction between animal and vegetal cells

Diffusible signal from vegetal half induces mesoderm

Isolated animal cap fails to form mesoderm and neuroectoderm