IN VITRO SELECTIVE AMPLIFICATION OF DNA (PCR) Flashcards
1
Q
steps of PCR
A
-melting
-annealing
-extention
2
Q
melting T
A
100 degrees
3
Q
annealing t
A
Tm -5 (between 50 and 65)
4
Q
extention T
A
70
5
Q
RT PCR amplification of… into
A
RNA
cDNA
6
Q
non specific fluorence
A
STBR Green
7
Q
specific + indirect fluorence detection
A
taqman
8
Q
direct + specific fluorescence detection
A
light cycler
9
Q
for a pcr primer TM has to be… and %GC must be
A
-between 55 and 68
-45-55
