CLONING VECTORS Flashcards
1
Q
Features of a plasmid used as a cloning vector: (4)
A
-replication origin
-insertion site (polilinker or MCS)
-antibio resistance gene
-identification gene
1
Q
what is a phage genome made of ?
A
right arm and left arm + remplacement region
2
Q
left arm
A
phage structure
3
Q
right arm
A
bacteria lysis
4
Q
remplacement region
A
prophage pathway (recombinaison + exision)
5
Q
3 diffrent clonic vectors
A
YAC, BAC, Cosmid
6
Q
YAC
A
for yeast transformation, behaves like a eukaryotic chromosome
7
Q
BAC
A
for bacterial transformation, behaves like a plasmid
8
Q
Cosmid
A
plasmid containing cos sequences, encapsulated
9
Q
two ways of inserting DNA
A
recombination oor restriction enzyme
