ARTIFICIAL PLANT MUTANTS USED IN PLANT SCIENCE Flashcards
1
Q
what strategie is used ofr in planta function of a protein
A
from genome to phenotype by comparing wild types with knocked mutants
1
Q
two types of loss of function mutants
A
-knocked out
-knocked down
2
Q
selectable marker =
A
promotor
transcribed region
terminator
3
Q
transcribed region
A
codes for the resistance to AB and herbicides
4
Q
what is T DNA
A
piece of DNA incorporated in the functional gene
5
Q
what are RNA interference
A
small interfering RNAs + micro RNA to regulate genome expression we can amplify them to silence a gene
6
Q
what info can the analyse of mutants can give us (4)
A
about gene constructs and reporter proteins
7
Q
reporter genes
A
in the pomotor of the gene of interest
8
Q
main reporter genes
A
B gal and GFP (effect on GUS)
9
Q
A
