In Vitro Cloning - PCR

Question 1

PCR: 95°C

Answer 1

> Temperature first increased to 95°C to break hydrogen bonds and split the DNA into single strands

Question 2

PCR: 55°C

Answer 2

> Temperature then decreased to 55°C so that primers can attach

Question 3

PCR: 72°C

Answer 3

> Temperature increased to 72°C as that is optimum for DNA polymerase

> DNA polymerase attaches complementary free nucleotides and makes a new strand to align to each template

Question 4

Advantages Of In Vitro PCR

Answer 4

> Automated = more efficient

> Doesn’t require living cells

> Rapid production of copies of DNA