IMPREGNATION Flashcards
Mixture of highly purified paraffin and synthethic plastic polymers
Paraplast
Melting point of paraplast
56-57 C
Synthetic wax similar with paraplast
Embeddol
Melting point of embeddol
56-58 C
Less compressible than paraplast
Embeddol
Semisynthetic was recommended for embedding eyes
Bioloid
Product of paraffin containing rubber
Tissue mat
Ester wax melting point
46-48 C
Require heavy duty microfome
Ester wax
Example of heavy duty microtome
Slide or sledge microtome
Compatible with cellosolve and xylene
Ester wax
Polyethylene glycols
Carbowax
Melting point of carbowax
38-42 C
Purified form of nitrocellulose soluble solvents
Celloidin
For neurological tissues
Celloidin
for bones brain teeth and whole organs
Wer celloidin
Wet celloidin block is stored in
70-80% alcohol
For eyes
Dry celloidin
Dry celloidin is stored in
Gilsons mixture
Components of gilsons mixture
Chloroform
Cedarwood oil
More ecplosive
Low viscosity nitrocellulose
Tendency to crack in lvm prevented by
Plasticizer
- oleum ricini
- castor oil
Subjected to histochem and enzyme studies
Gelatin
For frozen specimen
Gelatin
Plastic resins
Epoxy
Polyester
Acrylic
Epoxy types
Bisphenol a
Epon
Spurr
Paraffin oven temp
55-60 C
Normally temp used for routine work with paraffin
56c
If the lab temp is 20-24 C
54-58 C
If the lab temp is 15-18 C
50-54 C
4 changes of wax 15 minutes intervals
Manual
Autotechnicon example
Elliott bench type processor
Parts of the autotechnicon
CSTTB
Cover plate Spring loaded plunger Timing disk Transfer arm Beaker
If there is presence of odor in the clearing agent?
Paraffin wax should be change
The first ethanol bath should?
Should be discarded
Clearing agent and dilute ethanols should be changed?
Atleast once a week