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HISTOPATHOLOGY > FIXATIVES > Flashcards

FIXATIVES Flashcards

1
Q

Satisfactory for routine paraffin section

A

Aldehyde fixatives

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2
Q

It allow the natural colors to be restored for one hour

A

70% alcohol

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3
Q

Methods of removing formalin pigment (brown)

A

Kardasewitch
Lillies
Picric acid

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4
Q

Prolonged storage of formaldehyde produce what?

A

Precipitation of white paraformaldehyde

Turbidity

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5
Q

White paraformaldehyde precipitates may be remove tru?

A

Add 10% methanol

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6
Q

Bleaching of tissues may be prevented by

A

Changing the fluid fixatives for 3 months

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7
Q

If fatty tissues are to be stored

What is added to prevent dispersion of fat into the fluid

A

Cadmium

Cobalt

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8
Q

Acid reaction due to formic acid formation may be nuetralized by adding?

A

Magnesium carbonate

Calcium carbonate

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9
Q

Added to buffer formalin but produces calcium deposits

A

Calcium acetate

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10
Q

For general post mortem tissue

A

10% formol saline

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11
Q

Surgical post mortem

A

10% NBF

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12
Q

Routine post mortem tissues

A

Formal corrosive

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13
Q

Silver impregnation

A

10% formol saline

Heidenhain’s susa

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14
Q

Best fixative do tissue containing iron pigments and elastic fibers

A

10% NBF

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15
Q

Silver reticulum method

A

Formal corrosive

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16
Q

Does not allow frozen tissue section

A

Formal corrosive

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17
Q

For glycogen and micro-incineration

Fix sputum

Coagulates mucus

A

Alcohol formalin

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18
Q

For enzyme histochem and EM

A

Glutaraldehyde

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19
Q

Trichrome staining excellent

A

Mercuric chloride

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20
Q

Routine fixative choice for tissue photography

A

Mercuric chloride

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21
Q

Recommended for renal tissues, fibrin, CT, muscles

A

Mercuric chloride

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22
Q

Mercuric deposits may be removed by

A

Saturated iodine solution in 96% alcohol

Alcoholic iodine solution

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23
Q

Mercuric chloride added by glacial acetic acid

A

Zenker’s

24
Q

Mercuric chloride added strong formaldehyde

A

Zenker formol

Helly’s

25
Q

It is added to the zenker fluid to prevent turbidity and formation of black precipitates

A

Glacial acetic acid

26
Q

For liver

A

Zenkers fluid

27
Q

For pituitary gland

A

Zenker formol

28
Q

For tumor biopsies

A

Heidenhain susa

29
Q

For bone marrow biopsies

A

B5

30
Q

Preserves carbohydrate

A

Chromic acid

31
Q

Preserves mitochondria

A

P. Dichromate

32
Q

For demonstration of chromatin
Mitochondria
Golgi bodies

RBC and colliod containing tissues

A

Regauds

Moller

33
Q

For tissue necrosis
Rickettsiae
Myelin

A

Orths fluid

34
Q

For acid mucopolysaccharides

A

Lead fixatives

35
Q

For demonstration of glycogen

A

Picric acid

36
Q

Removing of yellow color produced by picricacid

A

70% ethyl alcohol
Followed 5% sodium thiosulfate
Washed in running water

37
Q

For embryos and pituitary biopsies

A

Bouins soln

38
Q

Endometrial curettings

A

Bouins

39
Q

Not suitable for fixing kidneys

A

Bouins

40
Q

Messy

Excellent Fixative for glycogen

A

Brasils

41
Q

Solidifies at 17C

A

Glacial acetic acid

42
Q

Cause glycogen polarization

A

Alcohol fixatives

43
Q

Excellent for fixing dry and wet smear

A

Methyl alcohol 100%

44
Q

For fixing touch prep

A

Isopropyl alcohol 95%

45
Q

70-100 % concentration

A

Ethyl alcohol

46
Q

For lymph glands

Chromosomes

A

Carnoys

47
Q

For diagnosis of rabies

A

Carnoys

Acetone

48
Q

Most rapod fixative

A

Carnoys

49
Q

For mucopolysaccaridea and nuclear proteins

A

Newcomers

50
Q

Better reaction with feulgen stain

A

Newcomers

51
Q

Strong oxidizing solution

A

Osmium tetroxide

52
Q

Brilliant nuclear staining with safranin

A

Osmium tetroxide

53
Q

Depresses the staining power of hematoxylin

A

Flemming

54
Q

For phosphatases and lipases

A

Acetone

55
Q

For em

A

Glutaraldehyde
Osmic
Paraformaldehyde

HISTOPATHOLOGY (6 decks)

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