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HISTOPATHOLOGY > CLEARING > Flashcards

CLEARING Flashcards

1
Q

Process whereby alcohol or dehydrating agent is removed from tissue and replaced with substance that will dissolve wax

A

Clearing

De-alcoholization

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2
Q

Most commonly used clearing agent for de-alcoholization in the embeddinv process are:

A

Xylene
Dioxane
Chloroform
Cedarwood

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3
Q

Used when the tissue is to be cleared directly from water as in a frozen section

A

Glycerin

Gum syrup

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4
Q

Make the tissue transparent due to high refractive index for better visualization

A

Clearinh agents

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5
Q

Steps requiring complete clearing

A

Infilltration

Mounting

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6
Q

Generally suitable for most routine histologic processing shedules <24hrs <5mm thickness

A

Xylene

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7
Q

Miscible with alcohol and paraffin

A

Xylene

Toluene

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8
Q

Doesnt extract out aniline dyes

A

Xylene

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9
Q

Used for celloidin section

A

Xylene

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10
Q

Becomes milky when an incompletely dehydrated tissue is immersed in

A

Xylene

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11
Q

Substitute for xylene and benzene but slower

A

Toluene

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12
Q

Best clearing agent for embedding

A

Benzene

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13
Q

Can damage the bone marrow resulting to aplastic anemia

A

Benzene

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14
Q

Thick blocks up to 1 cm can be processed with this clearing agent

A

Chloroform

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15
Q

Clearing agent doesnt not become translucent

Doesnt make tissue transparent

A

Chloroform

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16
Q

Recommended for tough tissues

A

Chloroform

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17
Q

Toxic to the liver after prolonged inhalation

A

Chloroform

18
Q

Vapor attack the rubber seal

A

Chloroform

19
Q

For both celloidin and paraffin

A

Cedarwood oil

20
Q

Tissue with cedarwood oil initially float and may dry out before completely cleared

Prevented by?

A

Superimposing absolute alcohol of the surface of clearing agent

21
Q

For clearing embryos, insects, delicate specimen

A

Aniline oil

22
Q

Clear 70% alcohol without excessive shrinkage and hardening

A

Aniline oil

23
Q

Tendency to become adulterated

A

Clove oil

24
Q

Similar with chloroform but cheaper

A

Carbon tetrachloride

25
Q

Slow acting clearing agents

Used when double embedding is required

A

Methyl benzoate
Methyl salicylate
Amyl acetate

26
Q

Should not be used for nervous tissues and lymph nodes

A

Xylene

27
Q

Fast acting for urgent biopsies

A

Xylene

Benzene

28
Q

Tends to acidify partially filled vessel

A

Toluene

29
Q

Tissues tend to float with this clearing agent

A

Chloroform

30
Q

Extremely slow

A

Cedarwood oil

31
Q

Becomes milky when prolonged storage

A

Cedarwood oil

32
Q

For CNS and cytological studies

A

Cedarwood oil

33
Q

Slow and difficult

Expensive

A

Clove oil

34
Q

Faint pleasant odor

A

Terpineol

35
Q

Substitute for cedarwood oil

A

Terpineol

36
Q

For clearing skin and smooth muscle

A

Oil of bergamot

37
Q

Use for delicate tissue

A

Oil of wintergreen

Aniline oil

38
Q

Xylene substitute

A

Toluene (xylene and benzene)
N-butyl acetate
Limonene

39
Q

Nitrocellulose solvent

A

N-butyl acetate
Amyl acetate
Methyl salicylate
Methyl benzoate

40
Q

Obtained form citrus fruits

A

Limonene

HISTOPATHOLOGY (6 decks)

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