IMPREGNATION

Question 1

Most common impregnating medium

Answer 1

Paraffin

Question 2

For large hollow tissues

Answer 2

Celloidin

Question 3

For frozen tissues, histochemical, and enzyme studies

Answer 3

Gelatin

Question 4

It is the process of removal of clearing agent from the tissue and replacing it with the infiltrating media

Answer 4

Impregnation

Question 5

Impregnation a.k.a.

Answer 5

Infiltration

Question 6

Melting point of paraffin wax

Answer 6

56 C

Question 7

Incomplete impregnation has…

Answer 7

Airholes in tissue sections

Question 8

> 60 C of paraffin temperature causes…

Answer 8

brittleness, shrinkage, hardening; destruction of lymph tissue

Question 9

A paraffin wax substitute that has a mixture of pure paraffin and synthetic plastic polymer

Answer 9

Paraplast

Question 10

A paraffin wax substitute that is used for embedding of eyes

Answer 10

Bioloid

Question 11

A paraffin wax substitute that is harder than paraffin thus used with sliding/sledge-type microtome

Answer 11

Ester wax

Question 12

A paraffin wax substitute that has rubber

Answer 12

Tissue Mat

Question 13

A paraffin wax substitute that is less brittle, and less compressible

Answer 13

Embeddol

Question 14

Most common water soluble wax

Answer 14

Carbowax

Question 15

Wet celloidin

Answer 15

For bones, brain, teeth

Question 15

Dry celloidin

Answer 15

For whole eye section

Question 16

Complete impregnation:

Answer 16

No fingerprint marks on surface of tissue block

Question 17

Disadvantage of Nitrocellulose method

Answer 17

Explosive when dry

Question 18

Disadvantage of celloidin

Answer 18

May take up for days and weeks

Question 19

Advantage of paraffin

Answer 19

Very rapid (24 hours), sections are cut easily without distortion, permits many staining procedures