FIXATIVES Flashcards

1
Q

for routine Histopathologic techniques

A

Formaldehyde

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2
Q

traditional most common fixative

A

Formol saline

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3
Q

best general tissue fixative

A

10% NBF

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4
Q

ideal for silver impregnation

A

Formol saline

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5
Q

ideal for silver reticulum

A

Formol sublimate

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6
Q

has HgCl2

A

Formol sublimate

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7
Q

for GI tissues, bone marrow, prostate biopsies

A

Hollande’s

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8
Q

for microincineration techniques, fixes sputum

A

Gendre’s

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9
Q

for electron histochemistry, electron immunocytochemistry

A

Karnovsky’s paraformaldehdye/glutaraldehyde

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10
Q

acrolein in glutaraldehyde

A

Karnovsky’s paraformaldehyde/glutaraldehyde

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11
Q

powder in form

A

Paraformaldehyde

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12
Q

for ultrathin and electron microscopy

A

Paraformaldehyde

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13
Q

for acid mucopolysaccharide & mucin

A

Lead

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14
Q

Most common metallic fixative

A

Mercuric chloride

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15
Q

good general fixative and good for trichrome staining

A

Zenker’s

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16
Q

for pituitary gland, BM, blood containing organs, preserve cytoplasmic granules

A

Helly’s/Zenker-formol

17
Q

for bone marrow biopsies

18
Q

minimum cell shrinkage and tissue hardening due to counter-balance effect of acid and mercury

A

Heidenhain’s Susa

19
Q

precipitates all proteins, and preserves carbohydrates

A

Chromic acid

20
Q

for mitochondria, chromatin, mitotic figures, golgi bodies, RBC and colloid-containing tissues

A

Regaud’s

21
Q

for early degenerative processes and necrosis, demonstration of rickettsia and other bacteria

22
Q

preserves lipids, mitochondria at pH 4.5-5.2, cytoplasm, chromatin, and chromosome

A

3% potassium dichromate

23
Q

for glycogen preservation

A

Picric acid

24
Q

for embryo and pituitary biopsies

25
important for nuclear fixatives
Glacial acetic acid
26
Most rapid tissue fixative
Carnoy's
27
Fixing brain tissues and rabies diagnosis
Carnoy's
28
for dry and wet smears, BM smears, bacterial smears
100% methanol
29
Touch prep smears to be wright-stained
95% isopropyl alcohol
30
for enzyme studies, does not fix but preserve glycogen
70-100% ethanol
31
for mucopolysaccharides and nuclear CHONs
Newcomer's
32
Pale yellow powder in water
Osmium tetroxide
33
Most common osmic acid fixative
Flemming's
34
for cytoplasmic structures
Flemming's w/o glacial acetic acid
35
Weak decalcifying agent
Tricholoroacetic acid
36
used at cold temp, for water diffusable enzymes, brain tissues
Acetone
37
Thermal coagulation of proteins
Heat