FIXATIVES

Question 1

for routine Histopathologic techniques

Answer 1

Formaldehyde

Question 2

traditional most common fixative

Answer 2

Formol saline

Question 3

best general tissue fixative

Answer 3

10% NBF

Question 4

ideal for silver impregnation

Answer 4

Formol saline

Question 5

ideal for silver reticulum

Answer 5

Formol sublimate

Question 6

has HgCl2

Answer 6

Formol sublimate

Question 7

for GI tissues, bone marrow, prostate biopsies

Answer 7

Hollande’s

Question 8

for microincineration techniques, fixes sputum

Answer 8

Gendre’s

Question 9

for electron histochemistry, electron immunocytochemistry

Answer 9

Karnovsky’s paraformaldehdye/glutaraldehyde

Question 10

acrolein in glutaraldehyde

Answer 10

Karnovsky’s paraformaldehyde/glutaraldehyde

Question 11

powder in form

Answer 11

Paraformaldehyde

Question 12

for ultrathin and electron microscopy

Answer 12

Paraformaldehyde

Question 13

for acid mucopolysaccharide & mucin

Answer 13

Lead

Question 14

Most common metallic fixative

Answer 14

Mercuric chloride

Question 15

good general fixative and good for trichrome staining

Answer 15

Zenker’s

Question 16

for pituitary gland, BM, blood containing organs, preserve cytoplasmic granules

Answer 16

Helly’s/Zenker-formol

Question 17

for bone marrow biopsies

Answer 17

B5

Question 18

minimum cell shrinkage and tissue hardening due to counter-balance effect of acid and mercury

Answer 18

Heidenhain’s Susa

Question 19

precipitates all proteins, and preserves carbohydrates

Answer 19

Chromic acid

Question 20

for mitochondria, chromatin, mitotic figures, golgi bodies, RBC and colloid-containing tissues

Answer 20

Regaud’s

Question 21

for early degenerative processes and necrosis, demonstration of rickettsia and other bacteria

Answer 21

Orth’s

Question 22

preserves lipids, mitochondria at pH 4.5-5.2, cytoplasm, chromatin, and chromosome

Answer 22

3% potassium dichromate

Question 23

for glycogen preservation

Answer 23

Picric acid

Question 24

for embryo and pituitary biopsies

Answer 24

Bouin’s

Question 25

important for nuclear fixatives

Answer 25

Glacial acetic acid

Question 26

Most rapid tissue fixative

Answer 26

Carnoy’s

Question 27

Fixing brain tissues and rabies diagnosis

Answer 27

Carnoy’s

Question 28

for dry and wet smears, BM smears, bacterial smears

Answer 28

100% methanol

Question 29

Touch prep smears to be wright-stained

Answer 29

95% isopropyl alcohol

Question 30

for enzyme studies, does not fix but preserve glycogen

Answer 30

70-100% ethanol

Question 31

for mucopolysaccharides and nuclear CHONs

Answer 31

Newcomer’s

Question 32

Pale yellow powder in water

Answer 32

Osmium tetroxide

Question 33

Most common osmic acid fixative

Answer 33

Flemming’s

Question 34

for cytoplasmic structures

Answer 34

Flemming’s w/o glacial acetic acid

Question 35

Weak decalcifying agent

Answer 35

Tricholoroacetic acid

Question 36

used at cold temp, for water diffusable enzymes, brain tissues

Answer 36

Acetone

Question 37

Thermal coagulation of proteins

Answer 37

Heat