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HISTOPATHOLOGY > FIXATIVES > Flashcards

FIXATIVES Flashcards

1
Q

for routine Histopathologic techniques

A

Formaldehyde

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2
Q

traditional most common fixative

A

Formol saline

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3
Q

best general tissue fixative

A

10% NBF

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4
Q

ideal for silver impregnation

A

Formol saline

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5
Q

ideal for silver reticulum

A

Formol sublimate

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6
Q

has HgCl2

A

Formol sublimate

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7
Q

for GI tissues, bone marrow, prostate biopsies

A

Hollande’s

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8
Q

for microincineration techniques, fixes sputum

A

Gendre’s

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9
Q

for electron histochemistry, electron immunocytochemistry

A

Karnovsky’s paraformaldehdye/glutaraldehyde

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10
Q

acrolein in glutaraldehyde

A

Karnovsky’s paraformaldehyde/glutaraldehyde

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11
Q

powder in form

A

Paraformaldehyde

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12
Q

for ultrathin and electron microscopy

A

Paraformaldehyde

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13
Q

for acid mucopolysaccharide & mucin

A

Lead

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14
Q

Most common metallic fixative

A

Mercuric chloride

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15
Q

good general fixative and good for trichrome staining

A

Zenker’s

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16
Q

for pituitary gland, BM, blood containing organs, preserve cytoplasmic granules

A

Helly’s/Zenker-formol

17
Q

for bone marrow biopsies

A

B5

18
Q

minimum cell shrinkage and tissue hardening due to counter-balance effect of acid and mercury

A

Heidenhain’s Susa

19
Q

precipitates all proteins, and preserves carbohydrates

A

Chromic acid

20
Q

for mitochondria, chromatin, mitotic figures, golgi bodies, RBC and colloid-containing tissues

A

Regaud’s

21
Q

for early degenerative processes and necrosis, demonstration of rickettsia and other bacteria

A

Orth’s

22
Q

preserves lipids, mitochondria at pH 4.5-5.2, cytoplasm, chromatin, and chromosome

A

3% potassium dichromate

23
Q

for glycogen preservation

A

Picric acid

24
Q

for embryo and pituitary biopsies

A

Bouin’s

25
Q

important for nuclear fixatives

A

Glacial acetic acid

26
Q

Most rapid tissue fixative

A

Carnoy’s

27
Q

Fixing brain tissues and rabies diagnosis

A

Carnoy’s

28
Q

for dry and wet smears, BM smears, bacterial smears

A

100% methanol

29
Q

Touch prep smears to be wright-stained

A

95% isopropyl alcohol

30
Q

for enzyme studies, does not fix but preserve glycogen

A

70-100% ethanol

31
Q

for mucopolysaccharides and nuclear CHONs

A

Newcomer’s

32
Q

Pale yellow powder in water

A

Osmium tetroxide

33
Q

Most common osmic acid fixative

A

Flemming’s

34
Q

for cytoplasmic structures

A

Flemming’s w/o glacial acetic acid

35
Q

Weak decalcifying agent

A

Tricholoroacetic acid

36
Q

used at cold temp, for water diffusable enzymes, brain tissues

A

Acetone

37
Q

Thermal coagulation of proteins

A

Heat

HISTOPATHOLOGY (6 decks)

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