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MTAP 2 - HISTOPATH > Impregnation > Flashcards

Impregnation Flashcards

1
Q

Process of removing clearing agent

A

Impregnation

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2
Q

Filling in the space through impregnation is for

A

Making the tissues be firm for easy cutting

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3
Q

Most commonly used impregnating agent

A

Paraffin wax

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4
Q

Advantage of using Paraffin wax

A

RAPID
Compatible with any staining technique

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5
Q

Disadvantage of Paraffin wax

A

Prolonged Impregnation
- brittle tissues

Incomplete process
- retention of clearing agent

NOT FOR FATTY TISSUES

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6
Q

Impregnating method not used for FATTY TISSUES

A

Paraffin wax

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7
Q

Melting point of Paraffin wax

A

56 deg cel

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8
Q

Requires 4 changes of paraffin wax

A

Manual

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9
Q

Oven temperature under Manual method

A

55-60 deg cel

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10
Q

Interval every change of wax in Manual method

A

15 minutes

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11
Q

Requires 2-3 changes of wax

A

Autotechnicon

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12
Q

More rapid than Manual; requires agitation

A

Autotechnicon

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13
Q

Contains negative atmospheric pressure

A

Vacuum

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14
Q

Vacuum infiltrating method is used for

A

CS-BEL

CNS
Spleen
Brain
Eyes
Lungs

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15
Q

Melting point for Paraplast

A

56-58 deg cel

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16
Q

Paraplast is used for

A

Brain
Bones

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17
Q

Paraplast component

A

Paraffin + synthetic plastic polymer

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18
Q

Same with Paraplast but with diff MELTING POINT

A

Embeddol

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19
Q

Substitute for paraffin wax used in eye spx

A

Bioloid

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20
Q

Paraffin result with rubber

A

Tissue mat

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21
Q

Has low melting point which is 46-48

A

Ester wax

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22
Q

Does not require clearing if SOLUBLE IN ALCOHOL

A

Ester wax

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23
Q

Ester wax has harder paraffin so it requires

A

Heavy duty microtome

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24
Q

Substitute used in histochemical and enzymes

A

Carbowax

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25
Advantage of Carbowax
Does not require dehydration and clearing
26
Disadvantage of carbowax
Difficult to float-out
27
Difficulty in floating-out using carbowax is remedied by
Addition of SOAP/ 10% Polyethylene glycol
28
Other term for celloidin
Collodion
29
Component of Celloidin
4.8% Ether and alcohol
30
Advantage of Celloidin
Do not require heat
31
Disadvantage of Celloidin
Very slow acting Sections for photomicrography are difficult to obtain
32
Celloidin is used for what type of tissues
Large and hollow cavities (Bones, brain)
33
Wet celloidin is used for what spx
BBTW Brain Bones Teeth Whole organ
34
Solution used in Wet celloidin for storage
70% alcohol
35
Dry celloidin is used for
Eye specimen
36
Dry celloidin solution for storage
Gilson’s mixture
37
Gilson’s mixture is composed of
Chloroform Caedarwood oil
38
Highly explosive, used in thin section
Nitrocellulose method
39
LVN produces THICK/THIN section
Thin
40
Prone of tissue cracking due to LVN may be remedied by
Oleum ricini/castor oil
41
Requires 2-3mm thick tissue, rarely used
Gelatin
42
Gelatin infiltration method is used for
Frozen Histochemical Enzyme
43
10% gelatin + 1% phenol is for
24 hrs
44
20% gelatin + 1% phenol is for
12 hrs
45
Addition of phenol is for
Preventing growth of tissues
46
Process of placing infiltrated tissue in embedding mold
Embedding
47
Other term for embedding
Casting Blocking
48
Requirement for embedding
Embedding mold Medium Infiltrated tissue
49
Temperature of melted paraffin used for embedding
5-10 deg above paraffin MP
50
Allows solidification of tissue
Refrigerator @-5 deg cel Cold bath
51
Cutting off excess paraffin wax to form FOUR-SIDED PRISM
Trimming
52
Infiltrating using celloidin, embedding using paraffin
Double embedding method
53
Non-disposable embedding molds
- Leuckhart’s - Compound - Plastic embedding rings and base molds - Tissue tek
54
With 2 L shaped strips of heavy/brass metal
Leuckhart’s
55
Produce blocks with diff sizes
Leuckhart’s
56
Embeds more than 1 tissue
Compound embedding unit
57
Produce perfect block w/o trimming
Peel-away
58
Economical and easy to prepare
Paper boat
59
Requires smearing of glycerine in inner part of mold
Plastic ice tray
60
Uses high resolution light microscopy
Plastic Resins Embedding
61
Epoxy are
Bisphenol Cyclohexene dioxide Glycerol
62
EM, seldom used Plastic Resin
Polyester
63
Used for LM (MMA, GMA)
Acrylic
64
Provides superior result for light microscopy
plastic (resins) emebedding
65
fastest epoxy resin
Spurr
66
Plastic resin originally introduced for EM
Polyester
67
Extremely hydrophilic (GMA)
Polyglyceryl methacrylate
68
Plastic resin considered ideal for undecalcified bones and hard tissues
Methyl methacrylate

MTAP 2 - HISTOPATH (5 decks)

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