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MTAP 2 - HISTOPATH > Decalcification > Flashcards

Decalcification Flashcards

1
Q

Potent anticoagulant

A

EDTA (vercene)

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2
Q

Known as weak decalcifying agent

A

EDTA

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3
Q

Chelating agent

A

binds with calcium
Weak dissociated complexes

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4
Q

Decalcifying acid used for surface decalcification of tissue

A

HCl

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5
Q

Most rapid decalcification method

A

Electrophoresis

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6
Q

Electrophoresis principle

A

Attraction between Ca++ in Negatively charged electrodes

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7
Q

Known to be a decalcifying agent and tissue softener

A

Perenyi’s

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8
Q

Most common decalcifying agent

A

5-10% nitric acid

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9
Q

5-10% nitric acid is used as decalcifying agent for

A

Urgent biopsies
Small biopsies
Needle

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10
Q

Temperature range for decalcification

A

18-20 deg.cel.

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11
Q

Most reliable test for completeness of decalcification

A

X-ray radiologic

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12
Q

Detect smallest amount of Ca+++

A

X-ray

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13
Q

Opaque result in Xray means

A

Incomplete decalcification

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14
Q

Completeness test of decalcification that uses calcium carbonate

A

Bubble test

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15
Q

Duration of decalcification through ion exchange ion

A

> 14 days

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16
Q

Completeness test for decalcification that involves pricking, bending, and probing

A

Mechanical/Physical

17
Q

Least reliable completeness test

A

Mechanical/Physical

18
Q

Phloroglucin- nitric acid is known as

A

Most rapid nitric acid containing decalcifying agent

19
Q

Excellent Bone decalcifier for EM and enzyme histochemistry

20
Q

Disadvantage of using EDTA

A

Inactivate ALP

21
Q

Remedy for inactivation of EDTA

A

Magnesium chloride

MTAP 2 - HISTOPATH (5 decks)

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