Decalcification

Question 1

Potent anticoagulant

Answer 1

EDTA (vercene)

Question 2

Known as weak decalcifying agent

Answer 2

EDTA

Question 3

Chelating agent

Answer 3

binds with calcium
Weak dissociated complexes

Question 4

Decalcifying acid used for surface decalcification of tissue

Answer 4

HCl

Question 5

Most rapid decalcification method

Answer 5

Electrophoresis

Question 6

Electrophoresis principle

Answer 6

Attraction between Ca++ in Negatively charged electrodes

Question 7

Known to be a decalcifying agent and tissue softener

Answer 7

Perenyi’s

Question 8

Most common decalcifying agent

Answer 8

5-10% nitric acid

Question 9

5-10% nitric acid is used as decalcifying agent for

Answer 9

Urgent biopsies
Small biopsies
Needle

Question 10

Temperature range for decalcification

Answer 10

18-20 deg.cel.

Question 11

Most reliable test for completeness of decalcification

Answer 11

X-ray radiologic

Question 12

Detect smallest amount of Ca+++

Answer 12

X-ray

Question 13

Opaque result in Xray means

Answer 13

Incomplete decalcification

Question 14

Completeness test of decalcification that uses calcium carbonate

Answer 14

Bubble test

Question 15

Duration of decalcification through ion exchange ion

Answer 15

> 14 days

Question 16

Completeness test for decalcification that involves pricking, bending, and probing

Answer 16

Mechanical/Physical

Question 17

Least reliable completeness test

Answer 17

Mechanical/Physical

Question 18

Phloroglucin- nitric acid is known as

Answer 18

Most rapid nitric acid containing decalcifying agent

Question 19

Excellent Bone decalcifier for EM and enzyme histochemistry

Answer 19

EDTA

Question 20

Disadvantage of using EDTA

Answer 20

Inactivate ALP

Question 21

Remedy for inactivation of EDTA

Answer 21

Magnesium chloride