immunophenotypes of malignancies

Question 1

CD13+, CD33+

CD34-, HLA-DR-

Answer 1

APL (AML with t(15,17) PML-RARA)

Question 2

CD13+, CD34+, HLA-DR+

CD33-

Answer 2

AML with t(8,21) RUNX-RUNX1T1

Question 3

CD34+, CD117+, CD33+, CD13+, CD14+ and CD11b+

Answer 3

AML with inv(16) CBFB-MYH11

Question 4

CD33+, CD34+ and CD117+

Answer 4

AML with normal cytogenetics or complex karyotype

Question 5

tdt+, CD34+, CD10+

CD20-, CD33-

Answer 5

ALL with t(12,21) TEL-AML1

Question 6

tdt+, CD10+, CD19+

Answer 6

ALL with t(9,22) BCR-ABL1

Question 7

tdt+, CD19+

CD10-

Answer 7

ALL with t(v,11q23) x-MLL

Question 8

tdt+, CD3+, CD5+

CD10-, CD20-

Answer 8

T-ALL

Question 9

tryptase+, CD117+, CD25+

Answer 9

mastocytosis

Question 10

CD5+, CD23+
CD20-
monoclonal light chain restriction

Answer 10

CLL/SLL

Question 11

CD5+, CD19/20+, cyclin D1+
CD23-
monoclonal light chain restriction

Answer 11

mantle cell lymphoma

Usually t(11,14) cyclin D1-IgH

Question 12

CD10+, CD19+, CD20+

Answer 12

Burkitt’s lymphoma

Question 13

CD38+, CD138+

CD19-, CD20-

Answer 13

multiple myeloma

Question 14

BCL2+, CD10+, CD19+, CD20+, BCL6+

Answer 14

follicular lymphoma

diffuse large B-cell lmphoma

Question 15

CD30+, CD15+, Pax5+

CD20-

Answer 15

classic Hodgkin’s

Question 16

CD20+, Pax5+

CD30-, CD15-

Answer 16

nodular lymphocyte predominant Hodgkin’s

Question 17

CD3+, CD4+, CD5+

Answer 17

mycoses fungoides

Question 18

CD1+, CD3+, CD4+, CD5+, CD7+, CD8+ (but not all)

Answer 18

peripheral T-cell lymphoma NOS