Immunopharmacology I

Question 1

TLR1 & 2 dimer

(Ligand/PAMP, expression on which cells, where in the cell)

Answer 1

PAMP = lipopeptides (on gram positive bacteria)
expression = monocytes, dendritic cells, mast cells, eosinophils, basophils
where = on the plasma membrane

Question 2

TLR2 & 6 dimer

(Ligand/PAMP, expression on which cells, where in the cell)

Answer 2

PAMP = lipopeptides
expression = monocytes, dendritic cells, mast cells, eosinophils, basophils
where = on the plasma membrane

Question 3

TLR3

(Ligand/PAMP, expression on which cells, where in the cell)

Answer 3

PAMP = dsRNA, polyinosinic:polycytidylic acid
expression = macrophages and dendritic cells
where = endosome membrane

Question 4

TLR4

Answer 4

PAMP = LPS
expression = macrophages, dendritic cells, mast cells, eosinophils
where = plasma membrane

Question 5

TLR5

Answer 5

PAMP = flagellin (on gram negative)
expression =macrophages and dendritic cells
where = plasma membrane

Question 6

TLR7

Answer 6

PAMP = ssRNA
expression = plasmacytoid dendritic cells, macrophages, eosinophils, B cells
where = endosome membrane

Question 7

TLR8

Answer 7

PAMP = ssRNA
expression = macrophages and neutrophils
where = endosome membrane

Question 8

TLR9

Answer 8

PAMP = unmethylated CpG in DNA
expression = plasmacytoid dendritic cells, eosinophils, B cells, basophils

Question 9

gram positive bacteria

Answer 9

• have lipopeptides in their cell wall (no LPS)
• have DNA
• no outer membrane, so stain with crystal violet
• produce polymyxins against gram negative bacteria (bind to LPS and destabilize membrane)

Question 10

gram negative bacteria

Answer 10

• have lipopolysaccharides in their cell wall
• have flagellin
• have DNA
• have a second outer membrane so crystal violet dye does not penetrate (also means they’re harder to treat)