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605 Immunology > Immunology Lab 1 > Flashcards

Immunology Lab 1 Flashcards

1
Q

serum

A

plasma minus clotting factors/fibrin

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2
Q

plasma

A

blood without the cells

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3
Q

Tube for Plasma?

A

purple top (with anticoagulant like EDTA)

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4
Q

Tube for Serum?

A

red top; allow blood to clot

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5
Q

hemocytometer

A

instrument for counting the number of blood cells in a measured volume of blood

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6
Q

chamber factor

A

correction factor that takes into account the actual volume of cells you counted (typically 10^4)

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7
Q

How to calculate # of cells per mL of blood

A

cells in 1 square x (dilution factor) x (chamber factor)

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8
Q

How to calculate chamber factor, besides just knowing it’s 10^4?

A

1mm x 1mm x 0.1mm = 0.1mm^3 –> 0.0001 mL in volume, which is 10^-4, so correction is required to be 10^4

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9
Q

Reasons you may get an abnormal WBC count?

A

infection, allergy, cancer, or another immune-mediated disease

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10
Q

Differential count [of a blood smear]

A

used to obtain counts of the different WBC types (neutrophils, eosinophils, basophils, lymphocytes, monocytes) which can help determine the type of infection present

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11
Q

Increase in neutrophils?

A

bacterial infection

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12
Q

Increase in lymphocytes?

A

chronic infection, viremia, or immune-mediated

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13
Q

Increase in eosinophils?

A

parasitic infection or allergic response

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14
Q

Increase in monocytes?

A

acute or chronic bacteria/viral infection

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15
Q

Monocyte

A

horseshoe/kidney-bean shaped (unilobular) nuclei with pale cytoplasm

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16
Q

Neutrophil

A

segmented (multilobular) nucleus with diffuse pale cytoplasm, sometimes will see pale granules

17
Q

Eosinophil

A

segmented (bi/multilobular) nuclei, red/orange/pink granules in cytoplasm

18
Q

Basophil

A

segmented (multilobular) nuclei with dark blue/purple granules in cytoplasm

19
Q

Lymphocyte

A

overall a small cell with a nucleus taking up the majority of it, very thin rim of cytoplasm

20
Q
A

neutrophils

21
Q
A

eosinophils

22
Q
A

basophils

23
Q
A

lymphocytes

24
Q
A

monocytes

25
Q

What is important when comparing your counted WBCs to reference tables?

A

Units!!!

605 Immunology (14 decks)

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