Immunologial and molecular techniques Flashcards
1
Q
Transmission EM can only identify virus to which level?
A
Family
2
Q
Virus Isolation
A
Propagation of viruses w cell culture, eggs, bioassay
- relies on presence of infectious virus
- long process, demanding on skill and equipment
3
Q
Immunofluorescence and immunohistochemistry
A
- Virus or viral antigen is detected w anti-viral antibodies labelled w fluorophores or enzymes
4
Q
ELISA
A
detects specific antibodies in animals
5
Q
Haemagglutination inhibition assay
A
detects presence of antibody to a specific (haemagglutinating) virus
- serum containing antibody is added to known quantity of virus and RBCs. If specific antibody is present, HA is blocked
6
Q
Serology
A
- relies on availability of antibodies that recognize specific viral proteins
7
Q
Seroconversion
A
detection of change from abscence of specific antibodies to presence
8
Q
Viral neutralisation assay
A
- highly specific and sensitive
- presence of neutralising antibodies prevents CPE
