Immunohistochemistry Flashcards
1
Q
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What is IHC?
A
- A technique used to identify specific antigens within tissue sections using antigen-specific antibody
- standard tool used in research setting and diagnostics
2
Q
What is the IHC technique?
A
1.) Target antigen in the cell
2.Primary antibody attaches to target antigen
3. Secondary antibody attaches to primary antibody
4. Secondary antibody conjugates with enzyme horse radish peroxidase (HRP)
5. Chromogenic substrate diaminobenzidine (DAB) is added which is catalysed by HRP
6. Brown precipitate is formed which is visualised under a microscope
3
Q
What is the IHC protocol?
A
- Tissue fixation
- Antigen retrieval
- Blocking
- Primary antibody
- Secondary antibody conjugated with HRP
- Chromogen application
