Immunoassays

Question 1

Allows you to determine if a polyclonal serum sample collected from a patient has antibodies that bind specifically to a protein from a mixture of proteins

Answer 1

Western Blot

Question 2

Can only be used to determine if an antibody binds to a determinant on the surface of an indicator RBCS

Answer 2

complement fixation

Question 3

Requires the antigen: antibody binding and the antigen sample needs to contain a purified antigen and that the antibodies are monoclonal

Answer 3

ELISA

Question 4

Blood group cross matching test

Answer 4

indirect antiglobulin test

Question 5

Autoimmune conditions in which there is a production of autoantibodies that bind to patient’s own RBCs can be tested with

Answer 5

Direct comb’s test (DAT)

Question 6

Testing of an autoimmune condition resulting in production of IgG antibodies that have specificity for type 4 collagen can be confirmed by confirmation of IgG bound to glomerular basement membranes. What test?

Answer 6

Immunofluorescence

Question 7

CD40L is on what?

Answer 7

effector CD4 T cells

Question 8

CD40L binds to what where?

Answer 8

CD40 on B cells or macrophages

Question 9

Deficiency of CD3 would result in what?

Answer 9

near or total absence of T cells

Question 10

CD8 deficiency would result in patient being highly susceptible to?

Answer 10

viral and intracellular bacterial infections

Question 11

CD19 and CR2 can be found where?

Answer 11

both components of the B cell co receptor

Question 12

CD28 is found where?

Answer 12

on T cells

Question 13

CD28 binds to what?

Answer 13

B7 (on APCs)

Question 14

Deficiency of this would leave a patient highly susceptible to virtually all pathogens

Answer 14

CD28

Question 15

B7 is found where?

Answer 15

on APC