Immuno tools Flashcards

1
Q

Ouchterlany Assay (Double Diffusion)

A

Determine reactivity of Ag well with surrounding Ab

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2
Q

Hemagglutination Assay

A

Abs sense Ag coated RBCs

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3
Q

Direct Coombs

A

Detect Abs on pts RBCs via antihuman Ig = infants RBCs for RhD

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4
Q

Indirect Coombs

A

Detects Anti-Rbs Abs in Serum - Mothers serum

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5
Q

Elisa steps

A
  1. Target Ag
  2. pt serum
  3. label (anti-human Ig)
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6
Q

Direct Elisa

A

detects Ags

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7
Q

Indirect Elisa

A

detect Abs

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8
Q

Western Blot

A

HIV prots seperated, add pt serum, wash, add label

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9
Q

Complement Fixation

A

Assay for Ab and coat RBCs, add C - amt of lysis = complement activity

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10
Q

Direct ImmunoF

A

detects pts tissue Ags, used to ID autoreactive Ab

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11
Q

Indirect ImmunoF

A

detects pts serum Ab via anti-Ig - detects path/Ag specific Abs in pt serum (ANA)

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12
Q

Facs Analysis

A

scatter plots showing proportion of cells

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13
Q

Hemolytic Plaque assay

A

B cells with sheeps RBCs labeled with Ag - estimates Ab production of plasma cells

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14
Q

Elisaspot Assay

A

enumerates ag specific Ab producing B cells and amount of cytokine producing T cells

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15
Q

Lymphocyte Stimulation test

A

measure previous exposure of Ag - checks memory response - quantify proliferation of cells

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16
Q

Cytotoxicity Assay

A

tests T cell cytotoxic capabilities

17
Q

Mixed Lymphocyte Reaction

A

MHC 2 typing for transplants, if HLA dont match T cells proliferate and activate

18
Q

Microcytotoxicity

A

AntiHLA - Ab and C, imprecise measurement of MHC 1 differences of transplant, dye taken up looked at for matches