Immuno tools Flashcards
Ouchterlany Assay (Double Diffusion)
Determine reactivity of Ag well with surrounding Ab
Hemagglutination Assay
Abs sense Ag coated RBCs
Direct Coombs
Detect Abs on pts RBCs via antihuman Ig = infants RBCs for RhD
Indirect Coombs
Detects Anti-Rbs Abs in Serum - Mothers serum
Elisa steps
- Target Ag
- pt serum
- label (anti-human Ig)
Direct Elisa
detects Ags
Indirect Elisa
detect Abs
Western Blot
HIV prots seperated, add pt serum, wash, add label
Complement Fixation
Assay for Ab and coat RBCs, add C - amt of lysis = complement activity
Direct ImmunoF
detects pts tissue Ags, used to ID autoreactive Ab
Indirect ImmunoF
detects pts serum Ab via anti-Ig - detects path/Ag specific Abs in pt serum (ANA)
Facs Analysis
scatter plots showing proportion of cells
Hemolytic Plaque assay
B cells with sheeps RBCs labeled with Ag - estimates Ab production of plasma cells
Elisaspot Assay
enumerates ag specific Ab producing B cells and amount of cytokine producing T cells
Lymphocyte Stimulation test
measure previous exposure of Ag - checks memory response - quantify proliferation of cells