immobalised enzymes

Question 1

pros of isolated enzymes

Answer 1

less waste
more efficient
more specific
efficinet
less downstream processing

Question 2

pros and cons of immobalised enzymes

Answer 2

pro
reused
reliable
greater temperature tollerence in matrix
ease of manipulation

cons
reduced efficiency
higher inituial cost
more technical issues

Question 3

4 types

Answer 3

Adsorption
covalently bonded
lattice entrapment
membrane serperisation

Question 4

Adsorption

Answer 4

enzyme is mized with immobalising enzxymes and binds with ionic links
clay or porous carbon
enzyme leakage due to weaker bonds
simple and cheap

Question 5

covalently bonded

Answer 5

enzyme is bonded covaently to an inslouible support using cross linking agents
collage or clay
no enzyme leakage due to stonger bonds
ph and substatrete conc has very little effect
active site mayb be modified meaning it less effective

Question 6

lattice entrapment

Answer 6

enzyme is trapped in a matrix of netwrok of fibres
no binding forces so enzymes can be easily lost as it can be greatly affected by conditions
expensive
slow

Question 7

membrane enttrapment

Answer 7

a partially permeable membrane mayy seperate ezymes. slolute flows past while reactants diffuse through
wnzyme is bound not trapped
simple but expensive
slow