imaging live cells

Question 1

retinal photograph

Answer 1

Put light on eye -> retinal photograph
We can’t see cells
Red because light absorbed from haemoglobin

Question 2

point spread function

Answer 2

Typical eye has a lot of aberration
Bigger pupil = better picture
Deformable mirror: compensates for wavefront aberrations

Question 3

adaptive optics

Answer 3

Uses deformable mirror
Allows better imaging
Can now see cells/capillaries
Images need to be stabilised because blood flow through capillaries

Question 4

selective bleach method

Answer 4

identify cones

Question 5

type 1 diabetes

Answer 5

Growth of blood vessels
Microaneurisms

Question 6

neurovascular coupling in imaging

Answer 6

Flicker light: see vessels before vs after
Vessels change (DIAMETER)
THICKER

Question 7

breath hold

Answer 7

thicker capillaries

Question 8

Adaptive Optics Scanning Laser Ophthalmoscopy:

Answer 8

Light only comes from one point
Light focused on point of interest on sample
Light that scatters from this point -> measured with light sensitive detector
Light continually recorded (focus spot spans across in a pattern)
Focus light via confocal pinhole
Optical sectioning & higher contrast images

steps;
AO correction (through mirror)
Stimulus delivery (shine shape onto eye)
Stabilisation
Targeted stimulus delivery (from stabilisation and stim delivery at same time)