ILS Flashcards
What degree are most lab freezers held at?
-20 C
What degree are most lab fridges kept at?
1-6 C
Why would a freezer be lower than 20C?
storing tissue enzyme, or antibody samples
If alternative sample in freezer, what degree?
-80C
how often should temps be checked?
at least once daily
To deliver pipette
NO narrow 2 rings
blow out pipettes
2 narrow rings at the top
which pipette requires extra air to remove every last drop
blow out pipettes (2 narrow lines)
to contain pipette
same thing as blow out pipette
glassware for mixing
erlenmeyer and beakers
autoclave degree for 3 min
134C
autoclave degree for 15 min
121C
autoclave for prions
neutralized with 2M sodium hydroxide or concentrated formic acid
how often to calibrate pH meter
before use each day with 2 soln (neutral and acid, neutral and basic)
how a PH meter works
measures the difference in voltage created by the number of HYDROGEN ions dissociated in soln
what does the measuring pH electrode have in it?
silver based electrical wire suspended in a buffer of KCl or weak HCl (in a thin glass bulb with metal salts)
What does the reference electrode contain?
a wire of silver chloride, KCl or mercury Cl suspended in pH 7 KCl
how often must centrifuges be calibrated?
6 months or annually
parameters that water is graded on?
pH, particulate matter, silicates, resistivity, org material, microbio count
Distilled H20
prefiltered H20 from municipal sippy is boiled and the vapor is condensed and collected
distilled water dissolved solid amount
less than 10mg/L
distilled water electrical conductivity
1.1s/m or less (less than drinking, more than ultra pure)
double distilled
distilled water is distilled again
dI water
ion exchange resins, exchange H for minerals (sodium, calcium, irono, copper), pure but not sterile
reverse osmosis
semi-permeable member to remove large particles from drinking water. Uses pressure
carbon filtering
air filters and water. pebbles of carbon remove contaminants (chlorine, sediment, organic compounds, odor, and taste)
filtration
ultra filtration/microfiltration with pores 2 micrometers apart, virtually everything
type 1 lab water
most critical requirement, advanced analytical technique, DNA sequencing, etc, electrophoresis/blotting
type 2 lab water
buffers, ph soln, microbio media, feeds type 1 systems
type 3 lab water
lowest grade, for rinsing, heating baths, filling autoclaves, or to feed type 2/3 lab water systems
QNS
quantity not sufficient
units
KHDUDCMNP
PASS
Pull, aim, squeeze, sweep
RACE
rescue, alert, contain, evac
most common reagent in lab
h20
icteric
bilirubin excess
