ILS

Question 1

What degree are most lab freezers held at?

Answer 1

-20 C

Question 1

What degree are most lab fridges kept at?

Answer 1

1-6 C

Question 2

Why would a freezer be lower than 20C?

Answer 2

storing tissue enzyme, or antibody samples

Question 3

If alternative sample in freezer, what degree?

Answer 3

-80C

Question 4

how often should temps be checked?

Answer 4

at least once daily

Question 5

To deliver pipette

Answer 5

NO narrow 2 rings

Question 6

blow out pipettes

Answer 6

2 narrow rings at the top

Question 7

which pipette requires extra air to remove every last drop

Answer 7

blow out pipettes (2 narrow lines)

Question 8

to contain pipette

Answer 8

same thing as blow out pipette

Question 9

glassware for mixing

Answer 9

erlenmeyer and beakers

Question 10

autoclave degree for 3 min

Answer 10

134C

Question 11

autoclave degree for 15 min

Answer 11

121C

Question 12

autoclave for prions

Answer 12

neutralized with 2M sodium hydroxide or concentrated formic acid

Question 13

how often to calibrate pH meter

Answer 13

before use each day with 2 soln (neutral and acid, neutral and basic)

Question 14

how a PH meter works

Answer 14

measures the difference in voltage created by the number of HYDROGEN ions dissociated in soln

Question 15

what does the measuring pH electrode have in it?

Answer 15

silver based electrical wire suspended in a buffer of KCl or weak HCl (in a thin glass bulb with metal salts)

Question 16

What does the reference electrode contain?

Answer 16

a wire of silver chloride, KCl or mercury Cl suspended in pH 7 KCl

Question 17

how often must centrifuges be calibrated?

Answer 17

6 months or annually

Question 18

parameters that water is graded on?

Answer 18

pH, particulate matter, silicates, resistivity, org material, microbio count

Question 19

Distilled H20

Answer 19

prefiltered H20 from municipal sippy is boiled and the vapor is condensed and collected

Question 20

distilled water dissolved solid amount

Answer 20

less than 10mg/L

Question 21

distilled water electrical conductivity

Answer 21

1.1s/m or less (less than drinking, more than ultra pure)

Question 22

double distilled

Answer 22

distilled water is distilled again

Question 23

dI water

Answer 23

ion exchange resins, exchange H for minerals (sodium, calcium, irono, copper), pure but not sterile

Question 24

reverse osmosis

Answer 24

semi-permeable member to remove large particles from drinking water. Uses pressure

Question 25

carbon filtering

Answer 25

air filters and water. pebbles of carbon remove contaminants (chlorine, sediment, organic compounds, odor, and taste)

Question 26

filtration

Answer 26

ultra filtration/microfiltration with pores 2 micrometers apart, virtually everything

Question 27

type 1 lab water

Answer 27

most critical requirement, advanced analytical technique, DNA sequencing, etc, electrophoresis/blotting

Question 28

type 2 lab water

Answer 28

buffers, ph soln, microbio media, feeds type 1 systems

Question 29

type 3 lab water

Answer 29

lowest grade, for rinsing, heating baths, filling autoclaves, or to feed type 2/3 lab water systems

Question 30

QNS

Answer 30

quantity not sufficient

Question 31

units

Answer 31

KHDUDCMNP

Question 32

PASS

Answer 32

Pull, aim, squeeze, sweep

Question 33

RACE

Answer 33

rescue, alert, contain, evac

Question 34

most common reagent in lab

Answer 34

h20

Question 35

icteric

Answer 35

bilirubin excess