ILS Flashcards

1
Q

What degree are most lab freezers held at?

A

-20 C

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
1
Q

What degree are most lab fridges kept at?

A

1-6 C

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Why would a freezer be lower than 20C?

A

storing tissue enzyme, or antibody samples

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

If alternative sample in freezer, what degree?

A

-80C

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

how often should temps be checked?

A

at least once daily

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

To deliver pipette

A

NO narrow 2 rings

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

blow out pipettes

A

2 narrow rings at the top

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

which pipette requires extra air to remove every last drop

A

blow out pipettes (2 narrow lines)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

to contain pipette

A

same thing as blow out pipette

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

glassware for mixing

A

erlenmeyer and beakers

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

autoclave degree for 3 min

A

134C

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

autoclave degree for 15 min

A

121C

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

autoclave for prions

A

neutralized with 2M sodium hydroxide or concentrated formic acid

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

how often to calibrate pH meter

A

before use each day with 2 soln (neutral and acid, neutral and basic)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

how a PH meter works

A

measures the difference in voltage created by the number of HYDROGEN ions dissociated in soln

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

what does the measuring pH electrode have in it?

A

silver based electrical wire suspended in a buffer of KCl or weak HCl (in a thin glass bulb with metal salts)

16
Q

What does the reference electrode contain?

A

a wire of silver chloride, KCl or mercury Cl suspended in pH 7 KCl

17
Q

how often must centrifuges be calibrated?

A

6 months or annually

18
Q

parameters that water is graded on?

A

pH, particulate matter, silicates, resistivity, org material, microbio count

19
Q

Distilled H20

A

prefiltered H20 from municipal sippy is boiled and the vapor is condensed and collected

20
Q

distilled water dissolved solid amount

A

less than 10mg/L

21
Q

distilled water electrical conductivity

A

1.1s/m or less (less than drinking, more than ultra pure)

22
Q

double distilled

A

distilled water is distilled again

23
Q

dI water

A

ion exchange resins, exchange H for minerals (sodium, calcium, irono, copper), pure but not sterile

24
reverse osmosis
semi-permeable member to remove large particles from drinking water. Uses pressure
25
carbon filtering
air filters and water. pebbles of carbon remove contaminants (chlorine, sediment, organic compounds, odor, and taste)
26
filtration
ultra filtration/microfiltration with pores 2 micrometers apart, virtually everything
27
type 1 lab water
most critical requirement, advanced analytical technique, DNA sequencing, etc, electrophoresis/blotting
28
type 2 lab water
buffers, ph soln, microbio media, feeds type 1 systems
29
type 3 lab water
lowest grade, for rinsing, heating baths, filling autoclaves, or to feed type 2/3 lab water systems
30
QNS
quantity not sufficient
31
units
KHDUDCMNP
32
PASS
Pull, aim, squeeze, sweep
33
RACE
rescue, alert, contain, evac
34
most common reagent in lab
h20
35
icteric
bilirubin excess