IgM and IgG Flashcards
Classify the RBC groups as IgG or IgM; ABO, Rh, lewis, Ii, P, MNSs, Kell, Duffy, Kidd.
M, G, M, M, M, M, G, G, G
What are the 7 Common characteristics to IgMs?
Large Molecule, Binds Complement effectively, Direct agglutination at IS and room temp/colder is optimal, Naturally occurring, do not cross placentas and other than ABO are not clinically significant
What is different about Lewis antibodies?
They are mad by tissues and released into blood to be incorporated into the RBC membrane; like Velcro
What enzyme adds to what precursor to make Le?
PS—-(Fucosyl trasnferase)—> Le^a antigen
If you have Le, Se and H what is your lewis Gene?
Le(a-b+)
If you have Le, sese and H what is your lewis gene?
Le(a+b-)
If you have Le Se and hh what is your lewis gene?
Le(a+b-)
If you have lele, Se or se / h or H what is your lewis gene?
Le(a-b-)
Look at slide 14 and fill in
Review
What are two reasons for lewis not being involved in HDN?
IgM (do not cross placenta) and they are not developed at birth so there is nothing to attach to
What precursor is I acting on?
PS1
What is the expression of I and i on adult cells?
I is on adult
What is the expression of I and i on cord cells?
i is on cord cells
At what age is Ii heterozygous seen?
2
What are the main pathological diseases with Anti-i and anti-I?
Little i: Infectious monoculeosis
I: Micoplastic Pneumoniae (Cold hemagglutinin Disease)
What phenotype is P1 like?
A1-> P1, P and Pk antigens
What phenotype is P2 like?
A2-> P2, P and Pk antigens
Where are P ags found?
on RBC
When are P ags developed and when are they fully expressed?
Fully developed AFTER birth and will have full expression at age 7
What is used to neutralize anti P1 or anti-P2 in antibody identifications?
P substance from Pigeon droppings
What phenotype can make anti P1?
P2
What disease is associated with P antibody?
PCH
Where are M & N antigens found and when are they fully developed?
Glycophorin A; developed at Birth
How are M and N inherited, what are they referred to as?
Mary and Nacy are inherited as a packet, similar to Rh
What are the 7 characteristics of IgGs?
Red Cell Stimulated, small in size (no direct agglutination) can cross the placenta, Warm-37, Do NOT bind complement, need AHG to see, cause HTR
What is the most common phenotype in Kell?
K-K+, Kp(a-b+), Js(a-b+)
What are the ag frequencies with Kell?
K=9%, k=98.8%, Kpa 2%W, Kpa>99.9%, Jsa99.9W or 99% B
THIRD OR FIRST immunogenic ??? wha…..
IMMUNOGENEIC, she only gives us third
What is the McLeod Phenotype ag expression and what disease is this associate with?
weakened expression of all K ags, Chronic Granulomatous Disease
Compare Fy(a+b-) or Fy(a-b+) in Caucasians with Fy(a+b-) or Fy(a-b+) in AA with regard to genes producing the phenotype.
Look at slide 19 in PP
What is the correlation of Fy(a-b-) in AA?
Linked to resistance of malaria (plasmodium vivax)
What are Antibodies of Kidd “bad?” Why?
They are notorious for causing severe HTR, play hide and seek, usually seen with other abs and don’t like to play alone, only super responders make them, enhanced with enzyme treatment, bind complement
What antibodies commonly bind complement?
Kidd and Lewis
Correlation of U ag with S and s.
U is not found on S and s but is found on all other antigens of MNs
What are the three sources from which compatible RBCs can be obtained with clinically sig. antibody to high frequency ag is found?
Autologous, siblings and rare donor file
What is the blood group system associated with the HLA system?
Bg Ags
What blood group system is sex linked?
Anti Xga
Name three systems besides the ABO in which soluble antigens occur.
Lewis, Ii, P
Name three blood systems with significant racial variation.
Lewis, Kell, Duffy
Five antigens destroyed by enzymes
Fya, Fyb, M, N, S
What is HLA?
High leukocyte antigen
What is HTLA?
High Titer low avidity
