Idk Yet Flashcards

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1
Q

DNA replication

A

The copying of double stranded DNA and necessary for cell reproduction prior to cell division 

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2
Q

What is semi conservative?

A

The resulting DNA copies, each have one strand of parental DNA, and one newly constructed strand

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3
Q

Helicase

A

Separates DNA strands

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4
Q

SSB

A

Single strand binding proteins, or SSPs, are tetramers that coat the DNA this prevents the DNA strands from reannealing to form double stranded, DNA 

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5
Q

Primase

A

Creates RNA primers

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6
Q

Polymerase

A

Strings nucleotides together to form a DNA strand 
(Extends DNA chain)

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7
Q

Sliding clamp

A

proteins that helps hold the DNA polymerase onto the DNA strand during replication 

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8
Q

RNase H

A

removes the RNA primers that previously began the DNA strand synthesis 

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9
Q

Ligase

A

Links short stretches of DNA together to create one long, continuous DNA strand

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10
Q

Steps of DNA replication

A
  1. Strands are separated
  2. SSBs coat single strands
  3. Primaries synthesizes RNA primers. 
  4. Polymerase attaches nucleotide at three prime, end
    Synthesis begins in the 5 to 3 direction
  5. One strand synthesizes continuously, while the lagging strand synthesizes Discontinuously 
  6. RN, ace H removes RNA primers.
  7. DNA polymerase I fills the gaps where RNA primers were removed 
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11
Q

DNA pieces separated by RNA primers in lagging strand are called

A

Okazaki fragments

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12
Q

A
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13
Q

Why is primase necessary

A

Polymerase can only extend a nucleotide chain and not start one. Primates uses the original DNA as a template to create a short and RNA primer 

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