IDENTTIFICATION OF MOLDS

Question 1

• Filamentous fungi
• The basic structural unit is _________
• It has rigid cell wall and
  increases in length as a result of apical growth
  with mitotic division.
• Divided into cells by the
  development of cross-walls (septa)
• Reproduces by Conidia o Microscopic
  spores

Answer 1

molds - hypha

Question 2

• After initial inoculation and incubation,
  cultures should be examined for growth:
  ✔1st week: Every day � Rapid
  Growers
  ✔2nd week: Every 2-3 days
  ✔3rd – 4th week: Once a week
  Slow Growers
• It is important to immediately
  subculture observed growth to ensure viability
  and isolation of the organism.
• Observe growth on Mycosel agar.
• When colony is mature, carefully note.

Answer 2

macroscopic examination

Question 3

– kills any live microorganisms

Answer 3

phenol

Question 4

preserves fungal structures

Answer 4

lactic acid

Question 5

stains the chitin present in the fungal cell wall

Answer 5

cotton blue

Question 6

• Tease mount technique

Answer 6

Microscopic Methods for Examination of
Fungi

Question 7

This is the best method for the
microscopic examination of mold cultures

Answer 7

d. Slide Culture (RIDDELL) Technique

Question 8

Main advantage is that morphology
more intact versus other methods

Answer 8

d. Slide Culture (RIDDELL) Technique

Question 9

• Shape - spherical, pyriform, clavate,
  ellipsoidal
• Septation
• Size
• Color (Pigmented or hyaline)
• Wall texture
• How many types of conidia seen (Micro
  or macro)

Answer 9

Conidia

Question 10

________________ is a printed or
computer-aided device that aids the
identification of biological entities, such as
plants, animals, fossils, microorganisms, and
pollen grains

Answer 10

In biology, an identification key,
taxonomic key, or biological key