Identification Flashcards
Buccal cavity: Triangular, trilobate lips
Ascaris lumbricoides
Symptoms and Pathology - Larval migration: Eosinophilia (resembles Loeffler’s syndrome)
Ascaris lumbricoides
MOT/DS of A.Lumbricoides
Ingestion/Egg
Adult - w/ lateral wings or cephalic alae
Enterobius vermicularis
Pruritus ani
Enterobius vermicularis
Resembles Japanese lantern
Trichuris trichiura egg
Barrel-shaped w/ bipolar mucus plug
Trichuris trichiura egg
Slender or attenuated in the anterior
Resembles a whip
Trichuris trichiura adult
S/P of t.trichuria
Rectal prolapse (Heavy infection)
pudoc disease or mystery disease
Capillaria philippinensis
w/ bipolar mucus plugs that are not as protruded as T. trichiura egg
Guitar, peanut-shaped
Capillaria philippinensis egg
Male: w/ chitinized spicule (spicule sheath)
Female: have eggs in utero
Capillaria philippinensis adult
S/P of Capillaria philippinensis
Borborygmi = peculiar abdominal gurgling sound
Agent of laziness in poor white population and tropical anemia in Puerto Rico
Hookworms (Necator, Ancylostoma)
“Morula ball”
Hookworms (Necator, Ancylostoma) egg
S-shaped, w/ semilunar cutting plates
N. americanus adult
C-shaped, w/ 2 pairs of teeth
A. duodenale adult
1st stage larva of hookworms
Short and stout
Open mouth: feeding stage
Buccal cavity: long
Genital primordium: small or inconspicuous
Rhabditiform larva
2nd stage larva of hookworms
Long and slender
Closed mouth: nonfeeding stage
Sheath: (+)
Tail: Pointed
Filariform larva
MOT Skin penetration
Hookworms
Mazza Mora/Water sore or ground itch
Hookworms
Lab. Diagnosis of hookworms
- Stool exam
= Egg: Diagnostic stage - Harada Mori Culture technique
- Baermann funnel technique (Strongyloides)
Cochin-China diarrhea
Strongyloides stercoralis
Adult: in intestines
Larva: in muscles
Trichinella spiralis
Requires 2 host to complete its life cycle
Propagated by black and brown rats
Trichinella spiralis
Male: w/ conical papillae
Female: w/ club-shaped uterus
Trichinella spiralis adult
MOT of t.spiralis
Ingestion of meat
Diagnostic stage of Trichinella spiralis
Muscle biopsy
= Encysted larva
DS of Filarial Worms
Microfilaria
Vector-borne nematodes
Filarial Worms
Filarial Worms
DH: man (IS: ____)
IH: vector (IS: ___)
filiform larva/3rd stage larva
microfilaria
Lab. Diagnosis of Filarial Worms
Knott’s technique
Habitat - Lymphatics
Wuchereria bancrofti
Vector of Wuchereria bancrofti
Aedes
Anopheles
Nocturnal (10PM-2AM)
Wuchereria bancrofti
Vector - Mansonia
Brugia malayi
Vector of Loa loa
Chrysops
Tabanid (mango fly)
Diurnal periodicity
Loa loa
Vector - Simulium (black fly)
Onchocerca volvulus
Unsheathed microfilaria
Onchocerca volvulus
Longest nematode of man
Dracunculus medinensis
IH - Cyclops (copepods): aquatic crustacean
Dracunculus medinensis
MOT - Ingestion of crustacean
Dracunculus medinensis
Causes human eosinophilic meningoencephalitis
Angiostrongylus cantonensis
IH - Mollusks:
- Achatina fulica
- Japanese snail
- Pila luzonica (kuhol)
- Brotia asperata (suso)
Angiostrongylus cantonensis
Female: “Barber’s pole appearance”
Looking of whitish uterus to red digestrive tract
Angiostrongylus cantonensis adult
Causes Herring’s disease
Anisakis
1st IH Various marine crustaceans
2nd IH Smallest fishes
3rd IH Larger fishes
Anisakis
DH - Dolphins/whales
Anisakis
All Trematodes except blood flukes [Schistosomes]
- Egg:
- MOT:
- IS:
- IH:
- Flat, leaf like
- Hermaphrodite
- Egg: operculated
- MOT: ingestion
- IS: metacercaria
- IH: 2 IH
Schistosomes:
- Egg:
- MOT:
- IS:
- IH:
- Elongated and cylindrical
- Separate sexes
- Egg: Non-operculated
- MOT: skin penetration
- IS: Cercaria (fork-tailed)
- IH: 1 IH (snail)
Trematode Eggs:
a. Mature when laid (embryonated
b. Immature when laid (unembryonated):
a “CHOS”
- Schistosoma
- Heterophyes
- Opistorchis
- Clonorchis
b “PEFF”
- Paragonimus
- Echinostoma
- Fasciola
- Fasciolopsis
Life cycle of trematodes
Egg > Larva (Miracidium>Sporocyst>Redia> Cercaria>Metacercaria) > Adult
Habitat of trematodes
Liver
Small Intestines
Lungs
Liver: COFF
- F. hepatica
- F. gigantica
- C. sinensis
- O. felineus
Small Intestines
- F. buski
- E. ilocanum
- H. heterophyes
Lungs:
-P. westermani
Causes sheep liver rot
Fasciola hepatica
w/ cephalic cone
Leaf-like hermaphrodite
Fasciola hepatica adult
Infects cattle in the Philippines
Fasciola gigantica
Most important liver fluke of man
Clonorchis sinensis
Old-fashioned electric light bulb
Clonorchis sinensis
Largest fluke parasitizing man
Fasciolopsis buski
Hen’s egg shaped
Fasciolopsis buski
Smallest but deadliest fluke of man
Heterophyes heterophyes
Causes endemic hemoptysis
Paragonimus westermani
w/ thickened abopercular end opposite the operculum
Paragonimus westermani Egg
Resembles coffee bean
Paragonimus westermani adult
Most romantic parasite of man
Schistosomes
Male: w/ gynecophoral canal where the female is held
Schistosomes adult
- Demonstration of egg in feces, rectal or liver biopsies, and urine
- Faust and Meleney’s egg hatching technique
- Circumoval precipitin test (COP)
lab dx Schistosomes
- Human sp: slight allergic reaction
- Nonhuman sp: Swimmer’s itch
Schistosomes
Causes Katayama’s disease
Schistosoma japonicum
w/ small knob-like or recurved hook on one side
Schistosoma japonicum egg
Oncomelania quadrasi IH
Schistosoma japonicum
Smallest of the blood flukes
w/ lateral spine
Schistosoma mansoni egg
Vesical blood fluke causing bilharziasis or urinary schistosomiasis (hematuria)
w/ terminal spine
Schistosoma haematobium egg
Adult: flat and ribbon-like (masking tape)
1 IH only
Trematodes
Trematodes
= anterior attachment organ
= region of growth
= chains of segments or proglottids
Cestode body
Scolex
Neck
Strobila
Largest tapeworm of man
Diphyllobothrium latum
Causes Vit. B12 deficiency anemia
Diphyllobothrium latum
Scolex: None, no rostellar hooks
Taenia saginata
Scolex: w/ rostellum armed w/ 2 rows of large and small hooklets
Taenia solium
Smallest tapeworm of man
Hymenolepis nana
Scolex: w/ rostellum armed w/ a ring of 20-30 spines
Hymenolepis nana
w/ oncosphere enclosed in an inner membrane w/ 2 polar thickenings, but ***w/o filaments
Hymenolepis diminuta eggs
Mature & gravid proglottids: melon seed/pumpkin seed/rice grain-shaped
Dipylidium caninum eggs
Shortest tapeworm (only 3 segments: immature, mature, ripe/gravid)
Anti-P1
Echinococcus granulosus
Casoni (intradermal test)
Echinococcus granulosus dx test
Rhizopoda
Non-motile
Nonfeeding stage
Well-formed stool
Infective stage
Cyst
Rhizopoda
Motile
Feeding stage
Diarrheic/liquid stool
Destroyed by iodine
Trophozoites
Commensals except Entamoeba histolytica
Class Rhizopoda
True amoeba Peripheral chromatin (trophozoite and cyst) Chromatoidal bars (cyst)
Entamoeba
Other amoeba
No peripheral chromatin
No chromatoid bars
Endolimax/Iodamoeba
Endoplasm: w/ RBC but no bacteria
Entamoeba histolytica Trophozoite INTJ
1 pseudopod (finger-like)
Entamoeba histolytica Trophozoite
Motility: Unidirectional & progressive
Entamoeba histolytica Trophozoite
- Motility: several directions, sluggish
- Many pseudopods (blunt & rounded)
- Endoplasm: w/ bacteria, yeasts and cell detritus (dirty-looking)
Entamoeba coli Trophozoite –INTP
- Chromatoidal bars: coffin/rod/cigar/ sausage-shaped
- Mature cyst w/ 1-4 nuclei
Entamoeba histolytica CYST
- Chromatoidal bars: splinter-like/ witch-broom/whisked broom
- Mature cyst w/ 1-8 nuclei
Entamoeba coli CYST
Smallest amoeba
Endolimax nana
Associated w/ gum infection
Entamoeba gingivalis
Large iodine-staining glycogen vacuole
Iodamoeba butschlii
Iodamoeba williamsi
CYST
Opportunistic Amoeba
Naegleria, Acanthamoeba, Hartmanella
Major causative agent of PAM
Naegleria fowleri
Causes keratitis (from contaminated contact lens care solution)
Acanthamoeba
[Cyst]
- Lemon to oval-shaped
- Protrusion at end
- Nipple-like cyst
Chilomastix mesnili
Associated w/ Gay bowel syndrome [Trophozoite] -Pear-shaped -Bilaterally symmetrical -2 nuclei, each w/ a large karyosome, lying w/in the sucking disk -Falling leaf motility -Old man’s face/old man w/ eyeglasses [Cyst] -2-4 nuclei -Football-shaped
Giardia lamblia
Trophozoite stage only Pear-shaped 4-5 flagella Jerky motility Undulating membrane: aids in locomotion
Trichomonas
Causes Pingpong’s disease
Trichomonas vaginalis