ID Bacteria & Antibiotic Testing Flashcards

1
Q

What is the catalase test used for? How do you preform it?

A
  1. catalase positive organisms include: Staphlococcus, E. coli and Pseudomonas.
  2. A drop of hydrogen peroxide (H2O2) is added to a bacterial sample on a slide. If bubbles occur, the bacteria contain catalase enzyme.
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2
Q

T/F: The effectiveness of an antibiotic depends on multiple different factors including the ability of the antibiotic to reach the site of infection

A

True

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3
Q

T/F: Only G- grow on MacConkey agar

A

True! Only lactose fermenters are able to form (pink) colonies.
Non-lactose fermenters do not grow.

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4
Q

AntiB that kills bacteria is called..?
AntiB that prevents bacteria from multiplying is..?

A
  1. Bactericidal kills bacteria
  2. Bacteriostatic prevents bacteria from multiplying
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5
Q

What are good reasons to test for antiB susceptibility of bacteria?

A
  1. Helps us know what antiB to use
    - for how long
    - dosage
    - bacteria resistance
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6
Q
  1. Coagulase test is preformed on catalase ___ and gram ___
  2. Staphyloccus a____ is coagulase ___
A
  1. Catalase positive & G+
  2. Aureus is coagulase positive
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7
Q

How do you do a coagulase test? (3)

A
  1. Bacteria colony is emulsified in a drop if distilled water/saline to create a thick susp.
  2. Drop of rabbit plasma & stir w/sterile loop
  3. Positive reaction + clumping 5 to 20 seconds
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8
Q

Kirby Bauer test (agar diffusion method), what is it? (4)

A
  1. Paper disks impregnated w/particular type of antiB
  2. Agar plate is swabbed so that the entire plate will grow a “lawn”
  3. AntiB disks are placed at intervals on top of agar
  4. After 18-20hrs of incubation plate is examined
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9
Q

what agar is most commonly used for antiB testing?!

A

Mueller Hinton agar!!

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10
Q

Minimum inhibitory concentration (MIC) is the…

A
  • Lowest concentration of an antimicrobial that will inhibit the visible growth of a microorganism after o/n incubation
  • AKA lowest amount of meds needed to stop said bacteria from growing
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11
Q
A
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