hypothalamus part 2 Flashcards
What are the features of a hypothalamic stem cell?
Cell body near the ventricle
A projection which acts as scaffolding
What are hypothalamic radial glia called?
Tanycytes
What is an emerging idea about pomc and NPY neurons and hypothalamic stem cells?
That pomc and NPY neurons can be generated from stem cells to anticipate and/or respond to the changing needs of the body
Examples of some markers of stem cells
Pea3
GLAST1
Fgf10
Examples of a marker of a progenitor cell
Islet1
What is does lineage tracing do?
Identifies all the progeny of a single/group of cells
What is CreERT2 and how is it activated?
Cre recombinase fused to a mutant estrogen ligand binding domain
It’s activated by tamoxifen
What happens when CreERT2 is activated by tamoxifen?
The stop sequence will be recombined by Cre, thus the reporter will be stably reported in that cell and all its daughters
What did a central injection of FG1 induce in rodents? What needed to be addressed in the experiment?
Induced sustained remission of diabetic hyperglycaemia in rodents
-Does fgf1 give the same result as fgf2?
Wha further questions need to be asked before the conclusion of the paper can be proven
- Are the neurons restored after FGF infusion, or are they restored from FGF-responsive tanycytes?
- Is it clear that the action of FGF in the brain is limited to its effect on tanycytes/ARC neurons?
- Do similar cells exist in humans and could a similar mechanism operate to induce sustained remission of T2D?
What is Islet1 imperative for?
Necessary for hypothalamic pomc expression during zebrafish and mouse embryogenesis
What are the descendants of Fgf10 tanycytes? And how do we know?
Neurons of the arcuate nucleus
Lineage tracing- CreERT2
Why is it difficult to identify if there is an imbalance of pomc/neuropeptipe y neurons?
Cannot count POMC neurons while you’re alive
No established ‘normal’ amount of POMC neurons
No antibody against POMC or neuropeptide Y neurons yet
*Can count POMC neurons in zebrafish
