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1
Q

Infiltrated with CELLOIDIN then embedded with PARAFFIN

A

Double embedding:

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2
Q

Flotation waterbath:

A

45 to 50 C

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3
Q

To remove formalin pigments:

A

Picric acid

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4
Q

To remove mercurial deposits:

A

Iodine

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5
Q

Explosive when dry:

A

Picric acid

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6
Q

Function of alum in hematoxylin:

A

Mordant

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7
Q

Primary importance alum in of Frozen Sections:

A

RAPID DIAGNOSIS

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8
Q

Enzyme histochemistry:

A

Frozen section

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9
Q

Second best choice for routine cytologic examination after Phase contrast microscopy

A

Papanicolau

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10
Q

NOT SUITABLE for kidney structures:

A

Bouin’s

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11
Q

should never contain osmic acid/osmium tetroxide because it inhibits hematoxylin

A

Microanatomical fixatives

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12
Q

should contain glacial acetic acid due to its affinity for nuclear chromatin.

A

Nuclear fixatives

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13
Q

They should never contain Glacial Acetic Acid because it destroys the mitochondria and Golgi bodies.

A

Cytoplasmic fixatives
‘HORFF’
(Flemming’s without HAc, Regaud’s, Orth’s, Helly’s and formalin with post-chroming).

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14
Q

Manual paraffin wax infiltration and embedding: At least ____________________________ to ensure complete removal of the clearing agent from tissue. The specimen is then immersed in another fresh solution of melted paraffin for approximately 3 hours to ensure complete embedding or casting of tissue.

A

(4) changes of wax are required at 15 minutes interval

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15
Q

Cambridge/Rocking microtome: invented by

A

Paldwell Treffall

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16
Q

Bond between Best carmine and glycogen

A

Coulombic attraction/electrostatic bonds
hydrogen bonds,

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17
Q

Regressive staining, it involves a differentiation step

A

Routine H and E

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18
Q

Stains for the glomerular basement membrane:

A

PAS, Azocarmine stain

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19
Q

immediately after death, rubbery consistency

A

Postmortem clotting

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20
Q

friable, characterized by fibrin precipitation

A

Antemortem thrombi

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21
Q

Leadership:

A

DIRECTING

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22
Q

Embedding medium for EM is

A

Plastic

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23
Q

Pap’s consists of 3 stains:

A

Harris hematoxylin
OG 6
EA

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24
Q

was attached to tumor the Department of Labor and Employment pursuant to Executive Order No. 565.
Executive Order No. 565-A was issued, delegating the Presidential power of control over the PRC to the DOLE Secretary

A

PRC

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25
Q

ALCOHOL FIXATIVE CONCENTRATIONS; because less concentrated solutions will produce lysis of cells.

A

70% to 100%

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26
Q

are commonly used fixatives for nucleic acids

A

Ethanol
methanol
Carnoy’s solution

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27
Q

: physical agent similar in mechanism to vacuum oven (heat) and agitation to increase movement of molecules and accelerate fixation. It is also used to accelerate staining, decalcification, immunohistochemistry microscopy.

A

MICROWAVE

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28
Q

LAST ALCOHOL BATH FOR DEHYDRATION SHOULD BE ______________. A of blue discoloration of _____________ crystals will indicate FULL SATURATION WITH WATER. Alcohol is then discarded and changed with fresh solution.

A

PURE ETHANOL; COPPER SULFATE

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29
Q

contains bundles of very long, multinucleated cells with crossstriations. Their contraction is quick, forceful, and usually under voluntary control. STRIATED, VOLUNTARY

A

Skeletal muscle

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30
Q

also has crossstriations and is composed of elongated, often branched cells bound to one another at structures called intercalated discs that are unique to it. Contraction is involuntary, vigorous, and rhythmic. STRIATED, INVOLUNTARY

A

Cardiac muscle

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31
Q

consists of collections of fusiform cells that lack striations and have slow, involuntary contractions. NONSTRIATED, INVOLUNTARY

A

Smooth muscle

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32
Q

Three (3) major changes that are observed in the NUCLEUS:

A

PYKNOSIS
KARYORRHEXIS (segmentation and fragmentation) KARYOLYSIS (dissolution of the nucleus)

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33
Q

Four (4) phases or stages of CELL DEGENERATION:

A

CLOUDY SWELLING
FATTY DEGENERATION
CELL DEATH OR NECROSIS
CALCIFICATION.

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34
Q

is performed by a physician and may be obtained by:

• Needle biopsy: most frequently performed method
• Surgical biopsy
• Percutaneous (entering through the skin) TREPINE (small object used to remove circular section of tissue) biopsy (core of bone with accompanying marrow is obtained)

A

BM aspiration

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35
Q

Carbohydrate fixation:

A

ALCOHOLIC FIXATIVES

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36
Q

NEUTRAL BUFFERED FORMALDEHYDE OR FORMALDEHYDE VAPOR

A

Protein fixation

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37
Q

ALCOHOL BASED such as Rossman’s fluid or cold absolute alcohol

A

Glycogen fixation

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38
Q

: fixative of choice for TISSUE PHOTOGRAPHY

A

MERCURIC CHLORIDE

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39
Q

LIVER, SPLEEN, CONNECTIVE TISSUE FIBERS and NUCLEI

A

Zenker’s fluid

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40
Q

PITUITARY GLAND, BM, BLOOD-CONTAINING ORGANS SUCH AS SPLEEN AND LIVER

A

Zenker’s-formol (Helly’s)

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41
Q

TUMOR BIOPSIES ESPECIALLY SKIN

A

Heidenhain’s susa solution

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42
Q

CHROMATIN, MITOTIC FIGURES, GOLGI BODIES, RBC and colloid containing tissues

A

Regaud’s (Moller’s/Muller’s) fluid

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43
Q

study of degenerative process and early tissue necrosis, demonstrates rickettsia and other bacteria

A

Orth’s fluid

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44
Q

ACID MUCOPOLYSACCHARIDES

A

LEAD FIXATIVES

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45
Q

fixation of embryos and pituitary biopsies

A

BOUIN’S

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46
Q

Bouin’s is NOT SUITABLE FOR FIXING _________ structures, lipid and mucus

47
Q

Glacial acetic acid solidifies at

48
Q

CHROMOSOMES, LYMPH GLAND and URGENT BIOPSIES

A

Carnoy’s fluid

49
Q

fixing of mucopolysaccharides and nuclear proteins

A

Newcomer’s fluid

50
Q

most common and fastest decalcifying agent

A

NITRIC ACID

51
Q

decalcifies and softens tissues at the same time

A

PERENYI’S FLUID

52
Q

most ideal, most ideal, most sensitive for determining extent of decalcification

A

X-ray or radiological method

53
Q

Embedding medium for electron microscopy:

A

EPON (PLASTIC MEDIUM)

54
Q

Manual H and E staining:

A

REGRESSIVE STAINING

55
Q

Flotation water bath: 45 to 50C, approximately

A

6-10C lower than the mp of the wax

56
Q

: vegetable dye extracted from LICHENS

57
Q

: probably the oldest of all stains

58
Q

demonstrating MITOCHONDRIA

A

JANUS GREEN

59
Q

Stain for Helicobacter pylori

A

TOLUIDINE BLUE
CRESYL VIOLET ACETATE

60
Q
  • increase in size of an organ due to an increase in size of individual cells
A

Hypertrophy

61
Q
  • increase in size of an organ due to increase in number of cells RETROGRESSIVE CHANGES
A

Hyperplasia

62
Q
  • failure of an organ to reach mature size
A

Hypoplasia

63
Q
  • organ is represented only by mass of fatty or fibrous nodule
64
Q
  • ACQUIRED decrease in size of a normally sized organ
    DEGENERATIVE CHANGES
65
Q
  • change in size, shape and orientation of cell
66
Q
  • change from one adult cell type to another
A

Metaplasia

67
Q

-change to a more primitive or embryonic cell type

A

Anaplasia or Dedifferentiation

68
Q
  • continuous abnormal proliferation of cells MALIGNANT TUMOR
A

Neoplasia or Tumor

69
Q

Most rapid of the common freezing agents:

A

LIQUID NITROGEN

70
Q

General purpose fixative:

A

10% neutral buffered formalin

71
Q

Neutral buffered formol saline or formaldehyde vapor

A

Protein fixation

72
Q

Fixatives for nucleic acids:

A

Ethanol, methanol and Carnoy’s solution

73
Q

Amount of fixative used has been 10 to 25 times the volume of tissue to be fixed. Recently, the maximum effectiveness of fixation is noted to be _______ the tissue volume

74
Q

Recommended ratio of fluid to tissue volume for DECALCIFICATION is

75
Q

Amount of dehydrating agent should not be ______________the volume of tissue

A

less than 10 times

76
Q

: Low grade to high grade alcohol

A

Dehydration

77
Q

Absence of water:

A

WHITE anhydrous copper sulfate

78
Q

Presence of water:

A

BLUE anhydrous copper sulfate

79
Q

Clearing aka

A

Dealcoholization

80
Q

Automated tissue processor:

A

Fix, dehydrate, clear, and infiltrate

81
Q

Histochemical demonstration of ENZYMES

A

FROZEN SECTION

82
Q

is the most commonly used fluorochrome to demonstrate DNA and RNA in fresh or fixed tissues.

A

ACRIDINE ORANGE

83
Q

Calcium salts = black

A

Von Kossa’s silver method

84
Q

Red chromogen for peroxidases:

A

Aminoethylcarbazole (AEC)

85
Q

Brown chromogen for peroxidases:

A

Diaminobenzidine (DAB)

86
Q

Administrative investigation:

A

2 members of board + 1 legal officer

87
Q

Policies and guidelines for Med Tech Education:

A

CMO no. 14 s. 2006

88
Q

Accreditation of clinical lab for training MT interns:

A

CMO no. 6 s. 2008

89
Q

All fixatives can be used except those that contain osmic acid. Osmic acid (like Flemming’s) inhibits hematoxylin

A

Fixatives for H & E

90
Q

Primary/Basic/Nuclear stain

A

Harris hematoxylin

91
Q

Secondary/Counterstain/Acid/Cytoplasmic stain

92
Q

stains the nucleus

A

Harris hematoxylin

93
Q

stains the cytoplasm of mature cells (superficial cells)

94
Q

stains the cytoplasm of immature cells (parabasal, intermediate cells)

A

EA 36/50/65:

95
Q

FIRST PRESIDENT: “Emergence of the Profession” (1963-1967)

A

Charlemagne T. Tamondong

96
Q

“Professional Recognition” (1967-1970)

A

Nardito D. Moraleta

97
Q

“Legislative Agenda” (1970-1971, 1973-1976)

A

Felix E. Asprer

98
Q

“Legislative Agenda” (1970-1971, 1973-1976)

A

Felix E. Asprer

99
Q

“Celebration of the Practice” (1971-1973)

A

Bernardo T. Tabaosares

100
Q

“Career Advocacy” (1973)

A

Angelina R. Jose

101
Q

(Venerable OCA) “Educational Enhancement” (1977-1981)

A

Venerable C.V. Chua

102
Q

“Image Building” (1982-1991)

A

Carmencita P. Acedera

103
Q

“Beyond Expectations” (2007-2012)

A

Leila M. Florento

104
Q

FATHER OF PAMET:

A

CRISANTO ALMARIO

105
Q

PAMET was originally organized on

A

SEPTEMBER 15, 1963

106
Q

PAMET HYMN Music:

A

Francis Jerota Pefanco

107
Q

PAMET HYMN Lyrics:

A

Hector Gentapanan Gayares, Jr.

108
Q

Current PAMET President:

A

Ma’am Luella Vertucio

109
Q

Current PASMETH President:

A

Dean Jose Jurel Nuevo

110
Q

Board of MT Head:

A

Dr. Marilyn Barza

111
Q

Board of MT Head:

A

Dr. Marilyn Barza

112
Q

Board of MT Member:

A

Ma’am Leila Lani Florento

113
Q

PRC CHAIRPERSON:

A

Atty. Charito Zamora

114
Q

DOH SECRETARY

A

Dr. Teodoro Herbosa

HBR (5 decks)

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