How was the Human Genome Project Carried Out?

Question 1

The Human Genome Project was first coordinated by what institutions?

Answer 1

US Department of Energy and the US National Institutes of Health

Question 2

The ____ at Hinxton in the UK also became a major partner in sequencing the human genome, ultimately sequencing around one - third of the genome.

Answer 2

Wellcome Trust Sanger Institute

Question 3

What chromosomes were sequenced at Wellcome Trust Sanger Institute?

Answer 3

Chromosomes 1, 6, 9, 10, 11, 13, 20, 22 and X

Question 4

In the Human Genome Project, the ____ comprising a total of 16 institutions in the USA, Europe, China and Japan was required to carry out the enormous sequencing task.

Answer 4

Human Genome Sequencing Consortium

Question 5

In the Human Genome Project, three institutions provided the necessary complex computational analysis. This include?

Answer 5

National Center for Biotechnology Information (NCBI) at the National Institutes of Health

European Bioinformatics Institute (EBI) in Cambridge, UK

University of California, Santa Cruz (UCSC), USA

Question 6

In the Human Genome Project, the strategy used was a ____ in which the regions of chromosomes submitted for fragmentation. The type of DNA sequenced using this approach were the ones whose location in the genome had already been determined and were contained in ____.

Answer 6

Hierarchical shotgun method; Bacterial artificial chromosomes (BACS)

Question 7

In the hierarchical shotgun method, large stretches of DNA contained in ____ were sequenced.

Answer 7

Bacterial artificial chromosomes (BACs)

Question 8

Celera also began to sequence the genome in 1999, but using a different strategy known as the ___.

Answer 8

Whole genome shotgun approach

Question 9

This method of sequencing the human genome involves sequencing many overlapping DNA fragments in parallel and then using a computer software to assemble the small fragments into larger contigs &, eventually, chromosomes.

Answer 9

Whole genome shotgun approach

Question 10

This method of sequencing the human genome involves initially breaking up the entire genome into millions of small fragments, sequencing these pieces in no particular order and subsequently reassembling the chromosome sequence by a massive computer analysis on the basis of sequence overlaps.

Answer 10

Whole genome shotgun approach