Gene Identification

Question 1

In this process of identifying genes, protein is isolated and the partial sequence of its amino acids determined. This then allowed the synthesis of a corresponding series of oligonucleotide probes based on the genetic code which could be used to identify the complementary gene from a DNA library.

Answer 1

functional cloning

Question 1

If a gene ’ s protein product was known, the gene could be cloned by what process?

Answer 1

functional cloning

Question 2

In contrast to functional cloning, if the gene ’ s protein product was unknown, the gene could be cloned by what process?

Answer 2

Positional cloning

Question 3

The first step of this process is to chromosomally map the gene and then to identify candidate genes from that region. The correct candidate was identified by mutational analysis in patients w/ the disease trait.

Answer 3

Positional cloning

Question 4

The first step of positional cloning is to chromosomally map the gene and then to identify candidate genes from that region. The correct candidate was identified by ____ in patients w/ the disease trait.

Answer 4

mutational analysis

Question 5

Many genes have been identified by the automated DNA sequencing of the genome as part of the Human Genome Project followed by gene prediction analyses in which genes are recognized by the computerised detection of typical gene features such as ___.

Answer 5

Transcriptional/Translational initiation sequence
Transcriptional/Translational termination sequences

Question 6

The probable functions of these genes can often also be predicted, by ____ in w/c similarities are found between the sequences of newly identified genes and those of genes, proteins or protein domains already listed in the databases

Answer 6

Automated homology searches