How Biotechnology Works- Part 2 Flashcards
Who invented recombinant DNA technology in 1972
2 people
Stanley Cohen of Stanford Univ
Herbert Boyer of UCSF
Recombinant DNA technology or genetic engineering is a method that allows the ____ _ ___ in a ___ ___ to form a _____ ___
combination of genes
Test tube
Hybrid DNA
Allows the transfer of a specific gene for a desirable trait
____ _____ or ____ ______
Genetic engineering or modern biotechnology
Tool are available (5)
Join DNA fragments using DNA _______
Cutting genes at specific sites by using _____ enzymes
Gel ___________
Synthesize DNA or RNA using DNA or RNA _______
Multiply DNA using ________ ___ ______ (1983)
Ligase Restriction Electrophoresis Polymerase Polymerase chaine reaction (PCR)
___ and ____ (1972) demonstrated that ____ cuts at ______ site and produces cohesive or ____ ends
Mertz and Davis
EcoR1
specific
sticky
Cutting genes at specific sites
Some restriction enzymes cut DNA to produce ___ or ___ ends
Some produce ___ ends
complementary; sticky
blunt
Separates charged molecules based on charge (positive, negative, neutral) and size
Gel electrophoresis
In gel electrophoresis, charged molecules migrate on a medium to which ___ ___ is applied
electric current
In Polymerase chain reaction:
Amplifying parts of DNA by _____
Involves repeated ___ _______ at __ temperature, primer _____, and DNA _____
In vitro replication
DNA denaturation
High
Attachment
synthesis
Nobel Prize in chemistry co-winner (1993)
Kary Mullis
Filipino that is co-discoverer of DNA ligase
Dr. Baldomero Olivera
Cohen developed a plasmid with only _ EcoR1 site, having deleted __ sites
Then when this plasmid (pSC101) is cut with EcoR1, it will open up forming a ____ DNA ___ with ____ ends
1, 12
Linear, duplex
Sticky
What animal did Cohen-Boyer-Berg use for experiment
African clawed toad
