Genetic Engineering Flashcards
Genetic engineering of microorganism Example 1. \_\_\_\_\_\_ human insulin (HI) gene from human DNA 2. \_\_\_\_ HI gene in a \_\_\_\_ 3. Transfection or \_\_\_\_ 4. \_\_\_\_ of plasmid, \_\_\_\_ of gene
- Isolate
- Insert, vector
- Transformation
- Multiplication, expression
In plants Step 1. \_\_ and \_\_\_ 2. Gene \_\_\_ 3. Particle \_\_\_ Gene \_\_\_ to plant tissue S\_\_\_\_\_ R\_\_\_\_\_ 4. G\_\_\_ selection/testing \_\_\_\_ testing VARIETY
- Gene and vector
- Construct
- Bombardment
Transfer
Selection
Regeneration - Greenhouse
Field
When do we use genetic engineering
The trait to be introduced is ____ ________ in the _______ of the crop
The trait is ___ ________ __ ______ by conventional breeding methods
It will take a ___ ____ _____ to introduce and or improve such trait in the crop by conventional methods
not present, germplasm
Very difficult to improve
Very long time
GE of animals
- ____ ___ of interest
- ____ fertilized sheep’s egg and ___ with gene
- _____ injected egg into a _______ mother sheep
- The ______ sheep is born. When mature, It produces desire protein in its milk
- The desired protrin is purified and may one day be used as a ____ product for ____
- Identify gene
- Isolate, inject
- Implant, surrogate
- Transgenic
- Health, people
Applications of rec DNA tech
Production of ___ _______ such as industrials and health products by microorganisms plants and animals
_____ of traits of plant and animals, using __ engineering, gene ____
___ and _____ of designer foods
Use in basic ____
Basic tool in _______
Important sunstances Improvement, genetic, editing Design, production Research GENOMICS
Modern biotechnology generations
First wave: _____ traits
2nd: ______ traits
3rd: F____
4th: _____ resources
5th: Use of __ ______ tools
Agronomic Quality Factories Renewable Gene editing
Applications and strategies
\_\_\_\_ of new trait \_\_\_expression \_\_\_\_ of expression; antisense, RNAi \_\_\_\_\_ engineering Provide \_\_\_\_ of \_\_\_\_\_
Expression Over Repression Protein Proof, concept
Current applications of modern biotech for HEALTH
____ for diabetes
____ for treating cancer
____ vaccine
Insulin
Interferon
Hepatitis
HT is ____
GM is both __ and insect-protected (__)
Herbicide tolerant
HT, Bt
First GM crop commercialized in PH
Bt corn
Examples of GM crops (6)
Bt corn Ringspot virus-resistant papaya Moondust carnation Blue rose Transgenic glofish GM salmon
The first biopharm drug approved
anticlotting drug
First biotech product produced in milk of goats
Atryn
Second biopharm drug approved
Treatment for lysosomal acid lipase (LAL) deficiency
Produced in egg whites of transgenic chickens
Kanuma
GM salmon is from the growth hormone of what salmon
Chinook salmon
3 future biotech products
Bt eggplant
Golden rice
Long shelf life papaya with PRSV resistance
