HIV Flashcards
Has a marked preference that serves as receptor sites for the virus
CD4+
HIV genome Gene: Gag
Viral gene product: _______________
Codes for core
structural (group ags)
proteins
P24, p18, p15
HIV genome Gene: Polymerase
Viral gene product: ___________
Transcribes ssRNA into dsRNA
Reverse transcriptase
Protease
RNAse
Integrase
HIV genome Gene: Envelope
Viral gene product: __________
Binds to CD4 receptors for infection
gp160
HIV genome Gene: Envelope
Viral gene product: __________
Required for viral fusion of the cell
gp41
HIV+ CD4+ count
> 200 mL3
AIDS+ CD4+ count
<200 mL^3
Seronegative period of HIV
6-8 weeks:
First antibody detected in HIV
Anti-gp41
Most widely used screening for HIV
ELISA Indirect
Confirmatory test for HIV
Most widely used supplementary test for confirming reactive HIV ELISA Ab test
Western Blot Assay
(+) result of Western Blot
gp41 band appears alone or
envelope (gp41, gp120, or gp160) appears in
combination with another HIV band
Tests for Detecting HIV Gene
In situ hybridization
Filter hybridization
Southern blot hybridization – DNA
DNA amplification
PCR – test HIV RNA
Test performed for the diagnosis of typhoid fever
Widal Test
Widal Test Slide antigens:
O (somatic) and H (Flagellar)
Widal Test: O positive
Typhoid fever
Widal Test: O positive and H
Typhoid fever
Widal Test: H
Past infection, Convalescence (Recovery), Exposure,
and Recent infection
Widal Test: O, H, and PARA A
Typhoid and Paratyphoid fever
Widal Test: PARA A, B, and C
Paratyphoid fever
Test detection of specific IgM and IgG to Salmonella typhi.
Thypidot
Positive reaction for thypidot
Color formation
Thypidot
No color: IgG
No color IgM
No typhoid fever
Thypidot
colored: IgG
No color IgM
Previous infection
Thypidot
No color: IgG
Colored: IgM
Acute typhoid fever
Thypidot
Colored IgM and IgG
Middle stage infection
Significant Titer value for Weil felix test
> 160
P.vulgaris antigen in weil felix
OX-19, OX-2
P.mirabilis antigen in weil felix
OX-K
Most widely used test for rubella (quantitative)
HEMEAGGLUTINATION INHIBITION
Hepatitis A is also known as
Enterovirus 72