Histology stains Flashcards

1
Q

Hematoxylin

A

Blue

Cation, binds to acidic/anionic/basophilic groups (ie DNA, ribosomes)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Eosin

A

Pink
Collagen, cytoplasm
Eosinophilic, sometimes called “acidophili”

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

PAS (Periodic acid-Schiff’s

A

Recolorizes by oxidation

ie carbohydrates after periodic acid (glycogen, glycoproteins, proteoglycans)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Masson’s trichrome stain

A

Nuclei purple-black
Cytoplasm red
Collagen and mucin green

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Gomori trichrome

A

Proteins green (collagen, muscle)
Nuclei purple
Lipophillic red
ie muscle biopsies

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Silver

A

Delicate structures, many modifications

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Resorcin-fuchsin

A

Elastic fibers -> dark blue

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Wright’s blood

A

Eosin and meth blue
RBCs pink
Nuclei dark blue
Differential of WBCs

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Giemsa

A

Similar to Wrights

Chromosome spreads

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Feulgen reaction

A

Quantitative DNA -> magenta

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Metachromasia

A

Substrates change stain (ie GAGs take toluidine blue -> red)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly