Histology stains Flashcards
Hematoxylin
Blue
Cation, binds to acidic/anionic/basophilic groups (ie DNA, ribosomes)
Eosin
Pink
Collagen, cytoplasm
Eosinophilic, sometimes called “acidophili”
PAS (Periodic acid-Schiff’s
Recolorizes by oxidation
ie carbohydrates after periodic acid (glycogen, glycoproteins, proteoglycans)
Masson’s trichrome stain
Nuclei purple-black
Cytoplasm red
Collagen and mucin green
Gomori trichrome
Proteins green (collagen, muscle)
Nuclei purple
Lipophillic red
ie muscle biopsies
Silver
Delicate structures, many modifications
Resorcin-fuchsin
Elastic fibers -> dark blue
Wright’s blood
Eosin and meth blue
RBCs pink
Nuclei dark blue
Differential of WBCs
Giemsa
Similar to Wrights
Chromosome spreads
Feulgen reaction
Quantitative DNA -> magenta
Metachromasia
Substrates change stain (ie GAGs take toluidine blue -> red)