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Histology > Histology of tissues > Flashcards

Histology of tissues Flashcards

1
Q

The function of Objective lenses

A

Bends light to magnify the image

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2
Q

What is the Scanning Electron Microscope?

A

It is used to create a 3D image of a specimen’s surface as a focused beam of electrons is passed over the gold-coated surface.

The collection of electrons to make the image is done by a computer software

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3
Q

Purpose of Sectioning

A

A microtome is a machine with a thin steel or glass blade used to cut the solidified specimen.

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4
Q

Light Microscopy

A

This microscopy technique is used to shine light through specimens to examine it.

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5
Q

What are some drawbacks to using TEM?

A

Requires heavy metal staining/shadowing

Time-consuming and labor-intensive

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6
Q

What are the 2 interacting components in the tissue?

A

Cells and the Extracellular Matrix (ECM)

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7
Q

What is used to collect/ create an image in TEM?

A

Fluorescent plate collects the electrons that pass through the ultrathin specimen

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8
Q

What are the two types of electron microscopes?

A

Transmission Electron Microscope (TEM)

Scanning Electron Microscope (SEM)

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9
Q

What are the Mechanical components in light microscopy?

FaMsLs

A

Focus adjustments

Mechanical stage

Light source

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10
Q

What is the role of the extracellular matrix?

A

To support cells and the fluid that transports nutrients to the cells, and carries away their catabolites and secretory products.

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11
Q

Function of condenser

A

Concentrates the light through the specimen

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12
Q

Purpose of Staining

A

This is used to visualize structures in the cell easier.

Acidophilic stains are acidic stains that colorize basic (+ve charge or alkaline) structures in the cell

eg. eosin

Basophilic stains are used to colorize acidic (-ve charge)structures in the cell

eg. Hematoxylin

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13
Q

Purpose of mounting

A

Each section is applied to a glass slide

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14
Q

What is an Electron microscope?

A

Uses electron beam instead of light to examine specimens and create an image

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15
Q

What is a Transmission Electron Microscope?

A

It produces 2D images of an ultrathin section by capturing electrons that have passed through it

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16
Q

What is the maximum magnification of the Electron microscope?

A

150,000 X

17
Q

What are the 4 major tissue types in adults?

A

Connective

Muscle

Nerve

Epithelial

18
Q

What is the resolution of a light microscope?

A

~0.1 micrometers

(0.1 x 10-6)

19
Q

What is TEM useful for?

A

Studying the cells interior

20
Q

Purpose of clearing

A

Treatment with Xylene makes the specimen transparent

21
Q

List steps to prepare a specimen

Fred Designs Clean Ember Sections Mounting Stains

A
  1. Fixation
  2. Dehydration
  3. Clearing
  4. Embedding
  5. Sectioning
  6. Mounting
  7. Staining
22
Q

What are the optical components?

COO

A

Condenser

Objective lenses

Ocular lenses

23
Q

Purpose of embedding

A

Plastics such as paraffin are sometimes used for embedding because they allow thinner sections to be made as they provide support so the specimen isn’t too distorted from cutting.

24
Q

What is the realistic resolution of the Electron microscope?

A

2 x 10-9 m or 2 nm

25
Q

Function of ocular lenses

A

Further magnifies the image

26
Q

Purpose of Dehydration

A

Used to remove any water from the specimen using alcohol baths

27
Q

Define tissue

A

A collection of cells with similar function and structure

28
Q

Purpose of fixation

A

Alters the chemical composition of the specimen and slows deterioration by treating it with a chemical fixative ie formalin

Protein crosslinking and denaturation may take place

29
Q

What does resolution mean?

A

The ability for a lens to reveal two individual points as being separate

Histology (9 decks)

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