histology of the gingiva & gingival CT (lamina propria) Flashcards

1
Q

3 parts of gingival epithelium

A

oral, sulcular, junctional

  • *epithelium = surface, outer layer that covers the connective tissues (muscles, fibers, etc)
  • *all 3 parts are continuous, but different
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2
Q

oral epithelium, strata

A

-faces oral cavity and extends from gingival margin to mucogingival jxn

  1. strata basale: contact with CT - capable of cell division
  2. strata spinosum: spines
    * *cells shrink away from one another yet stay in contact via desmosomes
  3. strata granulosum: keratohyalin granules
  4. strata corneum: keratinized, no nuclei (ortho) or nuclei/incomplete (para)
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3
Q

oral epithelium - connective tissue attachments

A
  • light microscopy: see basement membrane of connective tissue
  • CT of lamina propriae (papillae) interdigitates with epithelial rete pegs
  • deep numerous epithelial rete pegs: increase surface area for better attachment
  • comprised of HEMIDESMOSOMES, lamina lucida, lamina densa, anchoring fibrils, collagen fibers
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4
Q

oral epithelium - CT attachment, on elec microscope

A
  • you see basal lamina epithelial cells and a whitish not as dense layer called lamina lucida
  • then a darker line, lamina densa
  • *ALL CT FIBERS CONNECT TO LAMINA DENSA
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5
Q

keratinized: ortho vs para

A

ortho: no nuclei
para: nuclei still in these flattened cells, incomplete keratinization

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6
Q

oral epithelium cell renewal

A
  • *10 days**
    1. lose ability to divide
    2. produce increasing amounts of protein and keratin
    3. lose ability to produce protein, keratin, and energy source
    4. becomes dehydrated/flat, lose nuclei, become filled with keratin, maintain desmosomes
    5. sloughed away into oral cavity as desmosomes degenerate
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7
Q

other cells of oral epithelium

A
  1. melanocytes: make melanin
  2. langerhans cells: macrophage like cell, regulate tissue function
  3. merkel cell: tactile sensory
  4. lymphocyte: inflammatory response
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8
Q

sulcular epithelium

A

-faces enamel from gingival margin to junctional epithelium
-non keratinized
strata:
1. basale
2. spinosum
3. intermediate - flat cells with nuclei adn organelles
4. superficial - flat cells with organelles, no nuclei, slough off cell into sulcular fluid

**probably 25-30 cells thick

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9
Q

sulcular epithelium - light microscopy, what do you see

A
  • reduced rete pegs (should be smooth in health)
  • relatively flat interface
  • basement membrane between epithelium and CT
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10
Q

junctional epithelium

A
  • collar like band of epithelium that surrounds the tooth
  • attached to both tooth and CT
  • few desmosomes between cells, few intercellular spaces
  • *thickest at coronal part (15-30 cell layers) and tapers as you go apical
  • flat cells with long axis parallel to long axis of tooth
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11
Q

jxnal epithelium - why so few desmosomes

A
  • cells move super fast (4 days to get across)
  • very dynamic, always moving and changing
  • lots of intercellular space
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12
Q

where is jxnal epithelium

A

usually overlaps CEJ

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13
Q

where is junctional epithelium thickest

A

at most coronal part (15-30 cell layers) and tapers as it goes apical

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14
Q

jxnal epithelium - cell renewal

A
  • *4 days**
  • mitotic activity in all layers
  • cells move towards adn along tooths surface in coronal direction
  • migrate at oblique angle, not 90deg like oral epithelium
  • continuously attached to tooth
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15
Q

JE - CT attachment

A
  • attaches epithelium to tooth surface (cementum)
  • comprised of hemidesmosomes, lamina lucida, and lamina densa
  • lamina densa adjacent to tooth
  • light microscopy: you see basement membrane, no rete pegs, flat interface
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16
Q

what does JE attach to?

A

either enamel or cementum — has hemidesomosomes, lamina densa, lamina lucida
(lamina densa adjacent to tooth)
NO DESMOSOMES

17
Q

gingival connective tissue

A
  • primary cell is fibroblast, which makes the primary protein, collagen
  • multiple collagen fibers are long like noodles – provides support/shape of gingiva and attachment of tissue to bone/tooth
18
Q

collagen

A

primary protein in gingival connective tissue, 60%

made by fibroblast

19
Q

gingival fibers - circular, gingivodental, transseptal

A
  1. circular: maintain contour and position of marginal gingiva; encircle gingiva in ring like fashion
  2. gingivodental: from gingiva to tooth, provide gingival support
  3. transseptal: across septum, from cementum of one tooth to cementum of adjacnet tooth, maintain relationship of adjacent teeth, protects interproximal bone
20
Q

circular fibers

A

maintain contour and position of marginal gingiva, encircles gingiva in ring link fashion

21
Q

gingivodental fibers

A

from gingiva to tooth, provides gingival support

22
Q

transseptal fibers

A
  • across septum/interproximal space
  • from cementum of one tooth to cementum of next
  • maintain relationship of adjacent teeth
  • protects interproximal bone
23
Q

alveolo-gingival fibers

A

from alveolus to gingiva; attach gingiva to bone

24
Q

periosteogingival fibers

A

attach gingiva to bone

25
Q

transgingival fibers

A
  • like circular, but all intertwined

- secure alignment of teeth in arch

26
Q

interpapillary fibers

A
  • from tissue in papilla to gingiva

- provide support for interdental gingiva

27
Q

intercircular fibers

A
  • like circle, but they skip a tooth

- secure alignment of teeth in arch

28
Q

intergingival fibers

A

-provide support and countour of attached giingiva

29
Q

how does papilla get swollen adn enlarged?

A

extracellular fluid, which cannot get in until collagen fibers start to break down bc they are held so tightly together

30
Q

gingival crevicular fluid

A

-from gingival sulcus
-consists of amino acids, plasmids, plasma proteins (including Ab’s), electrolytes
^^^but in a different ratio than serum
-minimal amounts in healthy gingiva bc more structured/ impermeable sulcular epithelium
-increased amounts when inflamed

31
Q

attachment level (loss)

A
  • histological: epithelium and CT; see where jxnal epithelium ends relative to CEJ but you cannot see exactly where enamel stops
  • clinical: based on measurement using fixed reference point–CEJ; distance from CEJ to base of pocket; pocket depth + distance from gingival margin to CEJ
32
Q

what does it mean if you cannot find CEJ with probe

A
  • jxnal epithelium is at or above CEJ

- this means no clinical attachment loss

33
Q

lamina propria

A

gingival connective tissue