histology of the gingiva & gingival CT (lamina propria) Flashcards
3 parts of gingival epithelium
oral, sulcular, junctional
- *epithelium = surface, outer layer that covers the connective tissues (muscles, fibers, etc)
- *all 3 parts are continuous, but different
oral epithelium, strata
-faces oral cavity and extends from gingival margin to mucogingival jxn
- strata basale: contact with CT - capable of cell division
- strata spinosum: spines
* *cells shrink away from one another yet stay in contact via desmosomes - strata granulosum: keratohyalin granules
- strata corneum: keratinized, no nuclei (ortho) or nuclei/incomplete (para)
oral epithelium - connective tissue attachments
- light microscopy: see basement membrane of connective tissue
- CT of lamina propriae (papillae) interdigitates with epithelial rete pegs
- deep numerous epithelial rete pegs: increase surface area for better attachment
- comprised of HEMIDESMOSOMES, lamina lucida, lamina densa, anchoring fibrils, collagen fibers
oral epithelium - CT attachment, on elec microscope
- you see basal lamina epithelial cells and a whitish not as dense layer called lamina lucida
- then a darker line, lamina densa
- *ALL CT FIBERS CONNECT TO LAMINA DENSA
keratinized: ortho vs para
ortho: no nuclei
para: nuclei still in these flattened cells, incomplete keratinization
oral epithelium cell renewal
- *10 days**
1. lose ability to divide
2. produce increasing amounts of protein and keratin
3. lose ability to produce protein, keratin, and energy source
4. becomes dehydrated/flat, lose nuclei, become filled with keratin, maintain desmosomes
5. sloughed away into oral cavity as desmosomes degenerate
other cells of oral epithelium
- melanocytes: make melanin
- langerhans cells: macrophage like cell, regulate tissue function
- merkel cell: tactile sensory
- lymphocyte: inflammatory response
sulcular epithelium
-faces enamel from gingival margin to junctional epithelium
-non keratinized
strata:
1. basale
2. spinosum
3. intermediate - flat cells with nuclei adn organelles
4. superficial - flat cells with organelles, no nuclei, slough off cell into sulcular fluid
**probably 25-30 cells thick
sulcular epithelium - light microscopy, what do you see
- reduced rete pegs (should be smooth in health)
- relatively flat interface
- basement membrane between epithelium and CT
junctional epithelium
- collar like band of epithelium that surrounds the tooth
- attached to both tooth and CT
- few desmosomes between cells, few intercellular spaces
- *thickest at coronal part (15-30 cell layers) and tapers as you go apical
- flat cells with long axis parallel to long axis of tooth
jxnal epithelium - why so few desmosomes
- cells move super fast (4 days to get across)
- very dynamic, always moving and changing
- lots of intercellular space
where is jxnal epithelium
usually overlaps CEJ
where is junctional epithelium thickest
at most coronal part (15-30 cell layers) and tapers as it goes apical
jxnal epithelium - cell renewal
- *4 days**
- mitotic activity in all layers
- cells move towards adn along tooths surface in coronal direction
- migrate at oblique angle, not 90deg like oral epithelium
- continuously attached to tooth
JE - CT attachment
- attaches epithelium to tooth surface (cementum)
- comprised of hemidesmosomes, lamina lucida, and lamina densa
- lamina densa adjacent to tooth
- light microscopy: you see basement membrane, no rete pegs, flat interface