Histology of Adipose Tissue Flashcards

1
Q

What are the main functions of adipose tissue?

A

Adipose tissue is a type of specialized connective tissue with several functions:
* Stores fat for energy use
* Provides insulation from the cold
* Provides padding around organs
* Brown adipose can generate heat for newborns
* Releases hormones like leptin and estrogen (synthetic organ)

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2
Q

What cell types make up adipose tissue?

A

Adipose tissue is mainly adipocytes but the tissue also contains:
* Fibroblasts
* Vascular endothelial cells
* Macrophages

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3
Q

Each adipocyte is surrounded by a thick, specialized ECM called the _

A

Each adipocyte is surrounded by a thick, specialized ECM called the basal lamina

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4
Q

The basal lamina surrounding the adipocytes contains type _ collagen

A

The basal lamina surrounding the adipocytes contains type IV
* The basal lamina supports adipocytes, which are otherwise susceptible to injury because of the scanty cytoplasm

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5
Q

Differentiate between white adipose tissue and brown adipose tissue

A

White adipose tissue stores fat for energy needs

Brown adipose tissue generates body heat by metabolizing fat

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6
Q

Both white and brown adipose tissue stores fat in organelles called _

A

Both white and brown adipose tissue stores fat in organelles called lipid droplets
* Each white adipocyte has one lipid droplet
* Brown adipocytes have many lipid droplets

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7
Q

What do white adipocytes look like?

A

Each white adipocyte contains a central lipid droplet containing fat; this pushes the cytoplasm and nucleus to the periphery of the cell
* Cell can increase 20-fold in diameter
* Stored fat is mainly in form of triglycerides and cholesterol esters

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8
Q

What do brown adipocytes look like?

A

Brown adipocytes are smaller than white, they have multiple smaller lipid droplets per cell
* More abundant cytoplasm
* Nucleus is in the center
* Brown color comes from high quantity of mitochondria

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9
Q

White adipocytes have a remarkable ability to expand and store energy in the form of TG in the adipose tissue and liver; however, white fat is associated with _ and _

A

White adipocytes have a remarkable ability to expand and store energy in the form of TG in the adipose tissue and liver; however, white fat is associated with heart attacks and strokes

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10
Q

The main fat storage hormone is _

A

The main fat storage hormone is insulin
* It gets released from the islet cells of the pancreas
* Then binds to white adipose cell receptors
* And it inactivates lipase to promote fat storage

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11
Q

If higher fat mass leads to higher leptin, then why don’t we lose our appetite when we gain weight?

A

Leptin resistance at the receptor level

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12
Q

Explain the involvement of cytokines in obesity

A

Adipocytes contribute to a state of inflammation by secreting cytokines and inflammatory modulators like plasminogen, activator inhibitor 1, monocyte chemoattractant protein 1, TNFa, IL-6, and IL-8
* Macrophages in the adipose tissue also releases pro-inflammatory cytokines

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13
Q

Leptin binds to _ in the brain and acts to _

A

Leptin binds to the hypothalamus in the brain and acts to reduce hunger and stimulate satiety

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14
Q

Fat mobilization is caused by the following hormones:

A

Fat mobilization is caused by the following hormones: epinephrine, norepinephrine, glucagon, growth hormone, cortisol
* These activate lipase to cause breakdown from TG –> fatty acids + glycerol
* Fatty acids then get used by muscle and cardiac tissue for energy
* Glycerol is taken up by the liver to make new glucose

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15
Q

List some common sites of white fat deposition

A

Subcutaneous fat: located beneath the skin
Visceral fat: located around visceral organs (intra-abdominal)
Breast fat: in breast tissue
Marrow fat: in bone marrow
Ectopic fat: abnormal deposition in the muscles, heart, liver, pancreas

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16
Q

Subcutaneous fat is located in the _ layer of the skin; subcutaneous fat is not related to heart disease and stroke and may actually be protective

A

Subcutaneous fat is located in the hypodermis layer of the skin; subcutaneous fat is not related to heart disease and stroke and may actually be protective
* Sex hormone differences make premenopausal women more likely to have subcutaneous fat around the hips, thighs, buttocks

17
Q

Visceral fat is located inside the _ and is associated with _

A

Visceral fat is located inside the intra-abdominal region and is associated with insulin resistance, metabolic syndrome, type 2 diabetes
* Men are more likely to have this central obesity
* After menopause women’s fat moves from subcutaneous –> visceral and increases cardiovascular risk

18
Q

Marrow fat is adipose tissue that resides in the _ , interspersed with the hematopoietic cells

A

Marrow fat is adipose tissue that resides in the bone marrow , interspersed with the hematopoietic cells
* These adipocytes are derived from mesenchymal stem cells

19
Q

Why does ectopic fat occur if fat should not normally be inside muscle cells or in the liver?

A
  1. When white adipocytes get too big for their blood supply, they release biochemical signs of stress (ie cytokines) –>
  2. Immune cells are called in –> induce more cytokine production and inflammation
  3. Cytokines interfere with insulin signaling and increase lipolysis
  4. Induces a redirection of FFA towards peripheral tissues like muscle, liver, pancreas, heart

Fatty deposition below epicardium, fibrosis next to myocardium

20
Q

Fatty infiltration of the liver is called _

A

Fatty infiltration of the liver is called hepatic steatosis

21
Q

Brown fat produces endogenous heat in a process called _

A

Brown fat produces endogenous heat in a process called thermogenesis
* Brown fat is most abundant in newborn babies (5% of their body weight)
* The little brown fat that we have as adults is around the neck, shoulders, vertebrae, over the kidneys

22
Q

How does brown fat differ from white fat?

A
  1. Brown adipocytes have multiple lipid droplets for fat storage
  2. More mitochondria to generate heat
  3. Capillary supply is rich to carry the heat to the body
23
Q

Heat generation (of brown fat) is accomplished by a unique mitochondrial membrane protein called _

A

Heat generation (of brown fat) is accomplished by a unique mitochondrial membrane protein called mitochondrial uncoupling protein 1 (UCP1)
* Normally the movement of protons across the mitochondrial membrane would generate ATP (oxidative phosphorylation)
* In brown adipocytes, this special mitochondrial membrane protein instead converts the proton movement to heat

24
Q

Obesity is generally defined as _ body fat percentage in men and _ in women

A

Obesity is generally defined as > 25% body fat percentage in men and >33% in women

25
Q

Obesity in adults is defined as a BMI greater than or equal to _

A

Obesity in adults is defined as a BMI greater than or equal to 30

26
Q

The research gold standard of measuring totaly percent body fat is _

A

The research gold standard of measuring body fat is underwater weighing
* Whole-body displacement plethysmography, DEXA, bioelectrical impedance analysis, anthropomorphic techniques are other methods

27
Q

To measure subcutaneous fat we can use _ or _

A

To measure subcutaneous fat we can use skinfold thickness or ultrasound