histo postlab 3 (original notes)

Question 1

A process by which we give COLOR to a section

Answer 1

Staining

Question 2

Routinely used stain

Answer 2

H & E stain

Question 3

Color of NUCLEUS when H&E stain is used

Answer 3

Blue

Question 4

Color of CYTOPLASM and EXTRACELLULAR MATRIX when H&E stain is used

Answer 4

Pinkish

Question 5

Logwood Tree

Answer 5

Hematoxylon Campechianum

Question 6

Mixture of Giemsa Stain

Answer 6

Methylene-Blue
Eosin

Question 7

Mostly used on methanol-fixed blood films

Answer 7

Giemsa Stain

Question 8

Giemsa Stain binds to pathogens including

Answer 8

Spirochetes
Trypanosome

Question 8

Used to identify and differentiate the bacteria

Answer 8

Gram Stain

Question 9

Used to identify ‘‘ACID FAST’’ bacteria

Answer 9

Ziehl Nielsen Stain

Question 10

Used to identify myelin

Answer 10

Luxol fast blue/ Cresyl Violet

Question 11

Myelin is stained what color when Luxol Blue/Cresyl Violet is used

Answer 11

Blue

Question 12

Other elements of the Nervous System is stained what color when Luxol Blue/Cresyl Violet is used

Answer 12

Pink or Violet

Question 13

A dye that is more soluble in fat than in water or alcohols

Answer 13

Oil red

Question 14

Used as a stain for NEUTRAL LIPIDS

Answer 14

Oil red

Question 15

This stain is mainly used to evaluate the type and amount of EC material like COLLAGEN, FIBRIN, MUSCLE AND ELASTIC FIBER

Answer 15

Trichrome Stain

Question 16

Has a principle wherein smaller dye molecules will penetrate and stain a tissue element, but whenever the larger molecule can penetrate the same element, the smaller molecule is replaced by it

Answer 16

Massons’s Trichrome Stain

Question 17

Stains carbohydrates magenta, including components of the basal lamina

Answer 17

Periodic acid Schiff (PAS)

Question 18

Used to distinguish elements of the EC matrix
-Stains fungi and bacteria

Answer 18

Alcian Blue

Question 19

Used to identify deposits of PROTEIN IN TISSUE

Answer 19

Congo Red

Question 20

Naturally occurring vegetable dye

Answer 20

Orcein Stain

Question 21

Percent of periodic acid in Orcein Stain

Answer 21

0.5%

Question 22

Minutes of periodic acid in orcein stain

Answer 22

5 mins

Question 23

Minutes of Orcein in orcein stain

Answer 23

30 mins

Question 24

Deposits silver, which looks black

Answer 24

Silver staining methods

Question 25

Useful for identifying single cells amidst a group

Answer 25

Silver staining method

Question 26

Doesn’t stain every cell uniformly

Answer 26

Silver staining method

Question 27

A VERSATILE DYE that colors the nuclei blue

Answer 27

Toluidine Blue

Question 28

Used for examining with ELECTRON MICROSCOPY

Answer 28

Toluidine Blue

Question 29

Used for the detection of Collagen

Answer 29

Van Gieson Stain

Question 30

Color of Nuclei, when Van Gieson Stain is used

Answer 30

Blue/Black

Question 31

Color of Collagen, when Van Gieson Stain is used

Answer 31

Red/Pink

Question 32

Color of Cytoplasm, Muscle, Fibrin, and RBCs when Van Gieson stain is used

Answer 32

Yellow

Question 33

Can detect the Ferrous from the Ferric Ions

Answer 33

Perl’s-Prussian Blue

Question 34

With deposits found in the LIVER and PANCREAS

Answer 34

Hemochromatosis

Question 35

With deposits in the liver SPLEEN and LYMPH NODES

Answer 35

Hemosiderosis