Histo 1 Intro Methods 2016.Microscope Flashcards
What is histology ? What is another name for it ?
What is the purpose of fixation ? What do they do ? what is dehydration, what does it do ? what is one of the most popular fixative ? What are others ?
Dehydration is simply the removal of water from aqueous-fixed tissue. Since most fixatives are aqueous, this step is necessary to prepare the tissue for embedding in non-aqueous media like paraffin. Alcohols are most commonly used in the laboratory for tissue dehydration, since they are miscible with aqueous fixatives like 10% formalin
.What is embedding a sample , and sectioning ? What is the purpose ? What is the most common section thickness ?
Most embedding is nonpolar and harddern to keep the sample stable enough to cut it .
what must be done in order to visualize a tissue sample ? what does a stain allow you to see ?
what are the three common stains is used ?
What is an example of an acidic dye ? What is it, and what does it bind to ? What is an example of a basic dye, what is it, and what does it bind ? What is hematoxylin?
Where do hematixoylin and Eosin bind to ? What are some preferential stains ?
What is the use of Hematoxylin and Eosin to be able to determine different cell structure in an example of Eosinophils and Basophils .
What is the purpose of the reporter genes? What does it allow us to see ?
What is the purpose of immunycotchemistry ? How does it work?
What is the purpose of hybridization techniques? How does it work?
What is an example of hybridization technique using prox1 in the cornea?
What is an artifacts ? What’s the importance of noticing the difference between a artifacts and something real ?
Who invented the light microscope? What is the visible range of the human eye
about 400-800
What is a bright field microscope, and how is it used ?
Whats the advantage of a phase contrast microscope? How does it work? What kind of tissue is able to see?
What is another word for light ?
How does a fluorescent microscope work ? What kind of light ? Is it a dark field or bright field ?What kind of stains are utilzed ?
What is the purpose of confocal microsocpe. ? What does it create ? What kind of stains are used ?
Think of cutting a ball, in many sections , which allows you to see 3D imgine.
What does Transmission electron microscope use instead of light? What is the resolution, and what can it see? What do common Tem stain do to a sample ? Specimen preparation and manipulations are similar to that of ?
How does Scanning Electron Microscope use ? Does it penetrate, what does it look at ? What section of the sampel does it look at ?
Whats the difference between TEM and SEM
What is an atomic force microsocpe ? What is it able to do ? Can it see living or dead tissue? What is it similar to ?
Overview of the microscopy summary .
