hIGH THROUGHOUT SCREENING

Question 1

•How do antibiotics work?

Answer 1

Bactericidal vs bacteriostatic

Target cell wall, dna synthesis, protein synthesis, cell membrane

Question 2

Antibiotic resistance factors

Answer 2

efflux

immunity and bypass
target modifications
inactivativing enzymes

Question 3

Conjugation

Answer 3

transfer of genetic material between bacterial cells of same or different species by direct cell-to-cell contact.

Question 4

WHO Priority Pathogens

Answer 4

campylobacter
salmonella species
shigella spp
staphylococcis aureus

Question 5

Antibacterial drug discovery Main approaches

Answer 5

Target-based design

Whole cell screening

Question 6

Target-based design

Answer 6

Pick target Design inhibitors screen inhibitor

Question 7

Ethical Responsibilities

Answer 7

Replacement RefinementReduction

Question 8

Whole-cell screening –

Answer 8

screen for inhibitors against whole bacterial cells. Advantage: if get activity, know it is active against organism. Disadvantage: if active, don’t know mechanism of action/target