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PARASIT MIDTERM > Hemoflagellates > Flashcards

Hemoflagellates Flashcards

1
Q

Hemoflagellates include the _____ and ____

A

leishmaniasis,trypanosomes

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2
Q

Hemoflagellates may infect the?

A

Blood
lymph nodes
muscles
reticuloendothelial system

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3
Q

It multiply in blood and tissue of humans

A

Hemoflagellates

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4
Q

In hemoflagellates, all species require an ______ intermediate host

A

arthropod

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5
Q

What are the basic structures of hemoflagellates?

A

Single flagellum, a nucleus and a kinetoplast
Blepharoplast and parabasal body
Axial filament/axoneme

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6
Q

What are the stages of Hemoflagellates?

A

Amastigote
Promastigote
Epimastigote
Trypomastigote

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7
Q

Size of amastigote?

A

5-3um

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8
Q

Appearance of Amastigote?

A

Round to oval

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9
Q

Features of Amastigote?

A

Kinetoplast present

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10
Q

Size of Primastigote?

A

9-15um

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11
Q

Appearance of Promastigote?

A

Long and Slender

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12
Q

It has a kinetoplast located in anterior end

A

Promastigote

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13
Q

It has a kinetoplast located anterior to the nucleus

A

Epimastigote

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14
Q

It has a kinetoplast located in the posterior end

A

Trypomastigote

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15
Q

It has an appearance of C,S,U seen in stained blood films

A

Trypomastigote

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16
Q

It has an undulating membrane extending half of the body length

A

Epimastigote

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17
Q

It has an undulating membrane extending entire body length

A

Trypomastigote

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18
Q

What are the stages that are extending from anterior end?

A

Promastigote
Epimastigote
Trypomastigote

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19
Q

It is known as old world cutaneous leishmaniasis

A

Leishmania tropica

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20
Q

Leishmania tropica locally known as:

A

Oriental sore
Delhi boil
Aleppo button
Forest yaws
Baghdad/Jericho boil

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21
Q

It is known as new world cutaneous leishmaniasis?

A

Leishmania braziliensis

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22
Q

It is american or mucocutaneous leishmaniasis

A

Leishmania braziliensis

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23
Q

It is known as visceral leishmaniasis

A

Leishmania donovani

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24
Q

Clinical diseases of Leishmania braziliensis

A

Espundia
Uta
Chiclero ulcer
Nasopharyngeal
Leishmaniasis

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25
It has an habitat that endothelial cells of the infected skin, capillaries, and within the cytoplasm of large phagocytic monocytes
Leishmania tropica
26
It has an habitat that mucocutaneous junctions, particularly the nasal septum, mouth and pharynx
Leishmania braziliensis
27
It has an habitat that Endothelial cells of the reticuloendothelial system: liver, spleen, bone marrow, and visceral lymph nodes, and fixed tissue macrophages
Leishmania donovani
28
The Lab diagnosis should be skin scrapings (Wright’s or Giemsa stain)
Leishmania tropica
29
It has a lab diagnosis of Impression smears or tissue sections prepared from active lesions
Leishmania tropica
30
Biopsies should be obtained from elevated, inflamed margins of lesion
Leishmania tropica
31
Puncture of the ulcerated part
Leishmania braziliensis
32
Demonstration of the parasite from smears prepared from lesion scrapings (particularly the edge)
Leishmania braziliensis
33
It has a lab diagnosis of Blood smears, lymph node aspirate, Bone marrow puncture
Leishmania donovani
34
What are the other laboratory methods in Leishmania species
Novy-Mcneal-Nicolle medium Montenegro test(in vivo skin test)
35
Lab diseases: Kala-azar fever,Dum-dum fever, death fever, tropical splenomegaly
Leishmania donovani
36
It has a direct microscopy that fluid aspirate beneath ulcer bed, skin biopsy of ulcer
Cutaneous Lesion
37
It is same with Cutaneous Lesion, mucosal scrapings
Mucocutaneous lesion
38
It has a direct microscopy that tissue aspirates, blood
Visceral lesion
39
Staining of Leishmania species
Giemsa Stain Wright’s stain Leishmanin’s Stain H&E stain
40
In Novy, Mcneal, Nicolle Medium, Rabbit blood agar slope consisting of:
2 parts of salt agar 1 part of defibrinated rabbit blood
41
In Novy, Mcneal, Nicolle Medium, The material is inoculated into the water of condensation and culture is inucubated at?
22-24 degree C for 1-4 weeks
42
Originally designed for cultivation of insect tissue culture cells
Schnelder’s Drosophila Medium
43
It contains Inactivated fetal bovine serum, Antibiotic, Antimycotic solution
Schnelder’s Drosophila Medium
44
Skin test used in Leishmania species
Montenegro skin test
45
In skin test, what is the diameter and hour?
(+) 5mm induration after 12 hours
46
Appearance in skin test of 5mm incubation after 12 hours
Wheal appearance
47
What are the other non specific tests in Leishmania Species
Aldehyde Test of Napier(Formol Gel Test) Antimony Test of Chopra
48
Amounts in using Aldehyde Test in Napier (Formol Gel Test)
1-2mL px serum + 1-2drops of 40% formalin
49
Amount used in Antimony Test of Chopra
4% urea stibamine + px serum
50
Result in Aldehyde Test of Napier
(+) jellificaton
51
Result in Antimony Test of Chopra
(+) Profuse flocculence
52
Dulton , 1902
Trypanosoma brucei gambiense
53
Stephen and Fantham, 1910
Trypanosoma brucei rhodesiense
54
Chagas , 1909
Trypanosoma cruzi
55
Known as West and Mild African sleeping sickness
Trypanosoma brucei gambiense
56
known as East African sleeping sickness
Trypanosoma brucei rhodesiense
57
South American Trypanosomiasis Chagas Disease
Trypanosoma cruzi
58
Trypanomiasis of Trypanosoma brucei gambiense
Gambian Trypanomiasis
59
Trypanomiasis of Trypanosoma brucei rhodesiense
Rhodesian Trypanosomiasis
60
It is the only one that demonstrate the amastigote in macrophage
Trypanosoma cruzi
61
Specimen used in T. brucei gambiense & rhodesiense
Blood Tissue aspirates Cerebrospinal fluid
62
T. brucei gambiense & rhodesiense: In Wet mount preparation this should barely visible
Trypomastigotes
63
Permanent staining in T. brucei gambiense & rhodesiense
Giemsa stain Romanowski stain (Wright’s stain, Leishmanin’s stain)
64
What are the antibody detection in Immunodiagnosis of T. brucei gambiense & rhodesiense?
Indirect hemagglutinin (IHA) Indirect Immunofluoresence (IFA) Enzyme-linked immunosorbent assay(ELISA) Card agglutinatiin trypanosomiasis test (CATT) Complement fixation test (CFT)
65
Specimen used in Trypanosoma cruzi
Blood, tissue aspirate
66
Tissue Culture of T. cruzi
NNN Medium
67
In T. cruzi, Xenodiagnosid involves laboratory-bred bug:
Triatomine bug
68
Bugs feces examind after?
3-4weeks
69
Detection against T. cruzi
Antibodies (IgG)
70
In T. cruzi, antigen can be detected in?
urine & sera of px w/ Chronic Chagas dse
71
(Leishmania history) He Discovered the kalaazar/Indian visceral leishmaniasis
Sir William Leishman
72
He was the first describe amastigotes in the ulcers of skin in a man in Brazil
Lidenberg & Paranhos in 1909
73
Named the species as L.braziliensis
Vianna (1991)

PARASIT MIDTERM (7 decks)

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